RP-HPLC测定面醑Ⅱ号中甲硝唑和氯霉素的含量

SIMULTANEOUS DETERMINATION OF TWO EFFECTIVE CONSTITUENTS IN MIAN SPIRITS Ⅱ BY RP-HPLC

目的 测定面醑Ⅱ号中甲硝唑、氯霉素两者的含量。方法 采用反相高效液相色谱法 ,NovaPakC18色谱柱 (3 9mm× 15 0mm ,4 μm) ,以氢化可的松为内标 ,甲醇 -乙腈 -水 (30∶2 0∶5 0 )为流动相 ;检测波长 2 6 1nm。 结果 一次进样可完成 2种不同化学性质组分的含量测定 ,甲硝唑在 9 6 0～ 4 8 0 0 μg·ml-1、氯霉素在 9 89～ 4 9 4 4 μg·ml-1浓度范围内 ,两组份内标峰面积的比值与其浓度呈良好的线性关系 ,平均回收率分别为 99 1% (RSD =1 6 3% ) ,10 0 9% (RSD =0 90 % )。结论 该方法简便 ,快速 ,准确 。

OBJECTIVE To separate and determine two constituents (metronidazole,chloramphenicol) in Mian spirits Ⅱ.METHODS RP-HPLC was used.Hydrocortisone was used as internal standard.Chromatographic conditions included:Nova-PakC 18 column (3.9 mm×150 mm,4 μm) and mobile phase consisting of methanol-acetonitrile-water(30∶20∶50);detective wavelength was UV 261 nm.RESULTS The calibration curves were linear in the range of 9.60 -48.00 μg·ml -1 for metronidazole 9.89 - 49.44 μg·ml -1 for chloramplenicol.The average recovery is 100.8% , RSD = 1.63% for metronidazole;The average recovery is 100.2% , RSD = 0.90% for chloramphenicol.CONCLUSION The method is simple,rapid,accurate and suitable for the usual quality control of Mian spirits Ⅱ.