氢氧化镧对大鼠慢性肾衰高磷血症的改善作用研究

Study on Improvement Effect of Lanthanum Hydroxide on Chronic Renal Failure Hyperphosphatemia in Rats

目的:研究氢氧化镧对大鼠慢性肾衰高磷血症的改善作用。方法:取大鼠建立慢性肾衰高磷血症模型,再随机分为模型组、碳酸镧组[0.3 g/(kg·d)]、碳酸钙组[4.2 g/(kg·d)]和氢氧化镧高、中、低剂量组[1.5、1、0.5 g/(kg·d)],每组10只。同日上午继续ig腺嘌呤0.2 g/(kg·d),下午ig相应药物,1周后停止给予腺嘌呤,连续给药22 d,另取10只正常大鼠作为正常对照组。对各组小鼠进行一般检查,末次给药后检测大鼠肾脏系数,血清中钙、磷、甲状旁腺素(PTH)、肌酐、尿素氮含量,以及肾脏病理学变化。结果:与正常对照组比较,模型组大鼠呈现慢性肾衰体征,肾脏系数和磷、PTH、肌酐、尿素氮含量均增加,钙含量减少(P<0.01),肾脏切片呈明显病理学特征。与模型组比较,碳酸镧组、碳酸钙组和氢氧化镧各剂量组大鼠慢性肾衰体征均改善,肾脏系数(除氢氧化镧高剂量组外)和磷(除碳酸钙组外)、PTH(除氢氧化镧低剂量组、碳酸钙组外)、肌酐(除氢氧化镧低剂量组、碳酸钙组外)、尿素氮含量均减少,钙含量和钙磷乘积(仅碳酸钙组)均增加(P<0.05或P<0.01),其余差异无统计学意义;肾脏切片病理学特征好转。结论:氢氧化镧可改善慢性肾衰高磷血症模型大鼠肾功能,降低血清磷含量。

OBJECTIVE： To study the improvement effect of lanthanum hydroxide on chronic renal failure （CRF） hyperphos- phatemia in rats. METHODS： CRF hyperphosphatemia rat model were induced and then randomly divided into model group, lan- thanum carbonate group [0.3 g/（kg.d）], calcium carbonate group [4.2 g/（kg.d）] and lanthanum hydroxide high-dose, medium-dose and low-dose groups [1.5, 1, 0.5 g/（kg, d）] with 10 rats in each group. They were given adenine 0.2 g/（kg, d） intragastrically in the morning, and then given relevant medicine intragastrically in the afternoon; a week later, they stopped taking adenine but con- tinued to take relevant medicine for 22 d. 10 normal rats were selected as normal control group. General examination was conduct- ed, and renal coefficient, serum contents of calcium, phosphorus, PTH, creatinine （Scr） and usea nitrogen （BUN） were detected after last medication as well as renal pathological change. RESULTS： Compared with normal control group, model group showed CRF sign, renal coefficient, the contents of phosphorus, PTH, Scr and BUN were increased, while the content of calcium was de- creased （P〈 0.01）; renal section showed obvious pathological characteristics. Compared with model group, CRF sign of rats were improved in lanthanum carbonate group, calcium carbonate group and lanthanum hydroxide groups. The renal coefficient （except for lanthanum hydroxide high-dose group）, serum contents of phosphorus （except for calcium carbonate group）, PTH （except for lanthanum hydroxide low-dose group and calcium carbonate group）, Scr （except for lanthanum hydroxide low-dose group and calci- um carbonate group） and BUN were all decreased, while serum content of calcium and calcium-phosphorucs product （only in calci- um carbonate group） was increased （P〈0.05 or P〈0.01）. There was no statistical significance in other difference. The renal sec- tion pathological characteristics were improved. CONCLUSIONS： Lanthanum hydroxide can improve renal function and reduce the level of serum phosphorus in CRF hyperphosphatemia model rats.