川芎嗪联合乙酰半胱氨酸对慢性应激大鼠外周促炎细胞因子和肠道微生态的影响

Effects of Ligustrazine Combined with N-acetylcysteine on Peripheral Pro-inflammatory Cytokines and Intestinal Microflora of Chronic Stress Rat

目的:考察川芎嗪联合乙酰半胱氨酸(NAC)对慢性应激大鼠外周促炎细胞因子和肠道微生态的影响。方法:将正常大鼠作为正常对照组,将慢性应激大鼠分为模型组、NAC组(60 mg/kg)和川芎嗪+NAC组(15 mg/kg+60 mg/kg),每组20只,ig相应药物15 d。记录各组大鼠一般检查情况,检测血清中白细胞介素6(IL)-6、IL-1β、肿瘤坏死因子α(TNF-α)、干扰素γ(INF-γ)水平,检测肠道菌群。结果:与正常对照组比较,模型组大鼠呈现慢性应激症状,血清中IL-6、IL-1β、TNF-α、INF-γ水平均增强;肠道中的大肠埃希菌和肠球菌增加,乳杆菌和双歧杆菌减少(P<0.05)。与模型组比较,NAC组和川芎嗪+NAC组大鼠慢性应激症状减轻,上述指标均改善(P<0.05),其中川芎嗪+NAC组效果优于NAC组(P<0.05)。结论:川芎嗪联合NAC可通过降低促炎细胞因子水平和改善肠道微生态来改善大鼠慢性应激状态。

OBJECTIVE： To investigate the effects of ligustrazine combined with N-acetylcysteine （NAC） on the peripheral pro-inflammatory cytokines and intestinal microflora of chronic stress rat. METHODS： Normal rats were included in normal control group, and chronic stress model rats were divided into model group, NAC group （60 mg/kg） and ligustrazine＋NAC group （15 mg/kg＋60 mg/kg）, with 20 rats in each group. They were given relevant medicine intragastrically for 15 d. The general inspection of rats were recorded in each group. Level of IL-6, IL-1β, TNF-α and INF-γ in serum were detected, and intestinal microflora was measured. RESULTS： Compared with normal control group, chronic stress symptom of rats were found in model group, and level of IL-6, IL-1β, TNF-α and INF-γ in serum were enhanced; Escherichia eoli and Enterococcus in intestine were increased, while Bacterium lacticum and Bacillus bifidus were decreased （P〈0.05）. Compared with model group, chronic stress symptom of rats were relieved in NAC group and ligustrazine＋NAC group, and above indexes were all improved （P〈0.05） ; ligustrazine＋NAC group was better than NAC group （P〈0.05）. CONCLUSIONS： Ligustrazine＋NAC can improve chronic stress of rats by decreas- ing pro-inflammatory cytokines and improving the intestinal microflora.