石油炼化废水对ANAMMOX的脱氮性能影响研究

Impact of Oil Refining Wastewater on Denitrification Performance during ANAMMOX

为探究石油炼化废水对ANAMMOX过程中脱氮性能的影响,对AAOB细菌混培物进行驯化试验,并利用MPN-PCR技术对驯化前后的主要菌群进行计数。结果表明,石油炼化废水添加比例为50%时,NH_4^+-N和NO_2^--N的平均去除负荷分别为3.535、5.442 kg/(m^3·d),平均理论COD差值为16.67 mg/L;石油炼化废水添加比例为100%时,NH_4^+-N和NO_2^--N的平均去除负荷分别为未添加的17.4%和62.5%。AAOB细菌混培物对石油炼化废水存在一定的适应性,当废水添加比例为50%时增加了系统的稳定性和有效脱氮效果,驯化前后AAOB和DNB菌群数目变化较大,并且对菌群生化活性的影响方式不同;尽管驯化过程中优势菌群逐渐发生变化,但二者的协同脱氮作用一直存在。

In order to explore the impact of oil refining wastewater with high ammonia nitrogen and refractory organics on denitrification performance during ANAMMOX, a reactor was established using AAOB mixed bacterial culture to conduct domestication experiment, and the MPN-PCR molecular biology techniques were used to count the main bacterial communities before and after domestication. The results showed that when the addition proportion of oil refining wastewater was 500/0, the removal loading rates of NH4^＋ - N and NO2^- - N were 3. 535 kg/（m^3 ·d） and 5. 442 kg/（ m^3 · d）, respectively, and the theoretical difference value of COD was 16.67 mg/L during stable operation of the system, The removal loading rates of NH4^＋ - N and NO2^- - N with an addition proportion of 100% were 17.4% and 62.5% with no addition, respectively. AAOB mixed bacterial culture had certain adaptability to oil refining wastewater. The stability and denitrification efficiency of the system were enhanced when the addition proportion was 50%, and the influence on the numbers of AAOB and DNB before and after domestication changed greatly, biochemical activities of the bacterial communities was different. The dominant communities changed gradually during domestication, but the synergistic denitrification effect of the both always existed.