摘要
MicroRNAs(miRNAs)are small noncoding RNAs,which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis.So far,many miRNAs detection methods require polymerase chain reaction(PCR)amplification following reverse transcription of miRNAs.These processes are complicated and time-consuming.In this work,we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF_4:Yb,Er upconversion nanoparticles.In this method,the fluorescence of NaYF_4:Yb,Er upconversion nanoparticles were functionalized as a reference standard,which can improve the accuracy of miRNA detection.On the basis of these findings,we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.
MicroRNAs（miRNAs）are small noncoding RNAs,which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and prognosis.So far,many miRNAs detection methods require polymerase chain reaction（PCR）amplification following reverse transcription of miRNAs.These processes are complicated and time-consuming.In this work,we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF_4：Yb,Er upconversion nanoparticles.In this method,the fluorescence of NaYF_4：Yb,Er upconversion nanoparticles were functionalized as a reference standard,which can improve the accuracy of miRNA detection.On the basis of these findings,we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.
基金
the National Natural Science Foundation of China(61301039,21205036,31270908,61271056, 31540018)
the Hunan Provincial Natural Science Foundation of China (13JJ4091)
the Scientific Research Fund of Hunan Provincial Education Department(13A003)
China Postdoctoral Science Foundation funded project(2014T70459)
the Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province[(2013) 448]
