不同产地及不同部位黑龙骨中杠柳毒苷质量分数变化

Changes of Periplocin Contents in Different Parts of Periploca forrestii from Different Origins

分析不同产地及不同部位黑龙骨中杠柳毒苷质量分数分布规律,为黑龙骨药材的合理利用提供理论依据.用高效液相色谱法对采自不同产地的28份黑龙骨样品进行杠柳毒苷质量分数测定.结果表明,不同产地黑龙骨茎部杠柳毒苷总质量分数由多到少为云南野生材料,贵州野生材料,重庆野生材料,四川野生材料.同一产地根木质部杠柳毒苷质量分数最高(0.31~2.24mg/g);根韧皮部、茎韧皮部、茎木质部质量分数次之(0.25~1.41mg/g,0.09~1.23mg/g,0.05~0.34mg/g);叶部杠柳毒苷质量分数最低(0.04~0.21mg/g).因此,还可以考虑使用茎韧皮部、茎木质部和根韧皮部入药,扩大药用资源.

Object：To analyze the distribution of periplocin in different parts of Periploca forrestii plants originating from different places so as to provide a basis for the rational use of this traditional Chinese medicinal plant species.Methods：High performance liquid chromatography（HPLC）was adopted to determine the periplocin contents of 28 samples of P.forrestii collected from different origins.Results：A comparison of the wild species samples from different origins showed that wild P.forrestii originating from Yunnan had the highest total periplocin content in the stem,followed in order by those from Guizhou,Chongqing and Sichuan.For P.forrestii plants from the same place of origin,the highest contents of periplocin were found in the xylem of the root（0.31-2.24mg/g）,followed by the phloem of the root（0.25-1.41 mg/g）,the phloem of the stem（0.09-1.23 mg/g）and the xylem of the stem（0.05-0.34 mg/g）,and the leaves of the plant had the lowest periplocin content（0.04-0.21mg/g）.Conclusion：It is also recommended that the stem phloem and xylem,and the root phloem of P.forrestii plants be used as medicine so as to expand our medicinal resources.