淫羊藿总黄酮和薯蓣皂苷元联合用药对自然围绝经期大鼠影响的实验研究

Components of Epimedium Flavonoids and Diosgenin for Natural Perimenopause Rats

目的:观察中药有效组分淫羊藿总黄酮、薯蓣皂苷元对围绝经期大鼠内分泌激素水平、卵巢病变等影响。方法:自然衰老围绝经期雌性SD大鼠30只,随机分为3组,各10只,分别是中药低、高剂量组,老年模型组,另设6月龄左右雌性SD大鼠10只为青年对照组。中药组给予中药复方有效成分8周,观察阴道涂片变化,检测大鼠血清雌二醇(E_2)、促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)和黄体生成素(LH)值,免疫蛋白印迹法检测卵巢凋亡蛋白半胱天冬酶(Caspase-3)、血管内皮生长因子(VEGF)蛋白含量变化。结果:与老年模型组比较,青年对照组阴道涂片中有核上皮细胞百分率、E_2水平明显较高,LH、FSH、Caspase-3、VEGF的水平明显较低,差异均有统计学意义(P<0.05,P<0.01);中药高剂量组阴道涂片中有核上皮细胞百分率、E_2、VEGF水平明显升高,Caspase-3的水平明显降低,差异均有统计学意义(P<0.05,P<0.01);中药低剂量组阴道涂片中有核上皮细胞百分率明显升高,差异有统计学意义(P<0.01)。结论:淫羊藿总黄酮、薯蓣皂苷元合用能升高血清E_2和卵巢VEGF水平,降低卵巢Caspase-3的表达,从而延缓大鼠围绝经期的进程。

Objective：To observe the effect of Chinese Medicine effective components epimedium flavonoids and diosgenin compound on endocrine hormone levels,ovarian diseases,etc.of natural perimenopause rats.Methods：Selected30 female natural aging SD perimenopause rats and divided them into three groups randomly,10 rats in each group.These groups were Chinese medicine low dosage group,Chinese medicine high dosage group and the elder model group.Selected other 10 six-month-old or so female SD rats as the youth control group.Rats in Chinese medicine groups were given effective components of Chinese medicine compound for eight weeks.Observed changes of vaginal smears and detected serum estradiol（E_2）,gonadotropin releasing hormone（GnRH）,follicle stimulating hormone（FSH） and luteinizing hormone（LH）.Adopted western blot method to detect content changes of ovarian apoptosis protein Caspase（Caspase-3） and vascular endothelial growth factor（VEGF） protein.Results：When comparing with elder model group,in youth control group,percentage of nucleated epithelial cells on the vaginal smears and E_2 level were evidently higher,levels of LH,FSH,Caspase-3,VEGF obviously lower,differences being significant（P〈0.05,P〈0.01）.In Chinese medicine high dosage group,comparing with elder model group,percentage of nucleated epithelial cells on the vaginal smears,levels of E_2 and VEGF were significantly raised;levels of Caspase-3 were declined obviously,showing significant differences（P〈0.05,P〈0.01）.And in Chinese medicine low dosage group,percentage of nucleated epithelial cells on the vaginal smears was increased evidently as comparing with elder model group（P〈0.01）.Conclusion：Epimedium flavonoids and diosgenin compound can elevate levels of E_2 and VEGF,and decrease expression of ovarian Caspase-3,so as to delay the process of Perimenopause in rats.