浓缩归脾丸对苯中毒并抑郁小鼠应激能力的影响

Concentrate Guipi Pills on Stress Ability of Mice with Benzene Poisoning Merged Depression

目的:观察浓缩归脾丸对苯中毒并抑郁小鼠的定时游泳总里程、行为学及缺氧存活时间等应激能力的影响。方法:将72只小鼠随机分成6组,即正常组、模型组、归脾丸高、中、低各剂量组和西药组,每组12只。除正常组外,其余组均采用熏苯14天,并随机刺激14天建立苯中毒并应激抑郁小鼠模型,在造模成功后第1天开始给药。正常组、模型组均给予生理盐水0.2 mL/只灌胃;归脾丸高、中、低剂量组分别按8、4、2 mg/(kg·d)灌胃;西药组给予利血生1.5 mg/(kg·d),鲨肝醇5 mg/(kg·d),配制成混悬液灌胃;每天1次,连续3周。检测小鼠3 min定时游泳总里程、行为学及缺氧存活时间等指标。结果:与模型组比较,正常组3 min定时游泳总里程和小鼠的行为得分显著较高,缺氧存活时间显著较长,差异均有统计学意义(P<0.05,P<0.01);归脾丸各剂量组及西药组3 min定时游泳总里程显著延长,归脾丸各剂量组的垂直得分和水平得分明显升高,归脾丸各剂量组及西药组均能延长苯中毒小鼠的缺氧存活时间,差异均有统计学意义(P<0.05,P<0.01);与西药组比较,归脾丸各剂量组3 min定时游泳总里程、垂直和水平得分升高的更明显,归脾丸中、低剂量组小鼠的缺氧存活时间更长,差异均有统计学意义(P<0.05)。结论:归脾丸可提高苯中毒并抑郁小鼠的应激能力并减轻其抑郁的程度。

Objective：To observe the effect of Concentrate Guipi Pills（CGP） on stress ability,including time swimming total mileage,ethology and anoxia survival time of mice with benzene poisoning merged depression.Methods：Divided 72 mice into normal group,model group,CGP groups with high,moderate and low dose,and western medicine group randomly,12 in each.Except normal group,other groups were given fumigating benzene and random stimulation for 2weeks to build models.Medicines were taken after the first day of successful modeling.Normal group and model group were given normal saline（NS） with the dose of 0.2 mL per mouse by gavage.CGP groups with high-,moderate-and low dose were given 8,4,2 mg/（kg·d）,respectively.Suspension of 1.5 mg/（kg·d） leucogen and 5 mg/（kg·d） batyl alcohol were given to western medicine group by gavage.Once a day,lasting for 3 weeks.Detected index of 3 minutes swimming total mileage,ethology,and anoxia survival time.Results：Compared with model group,scores of 3 minutes swimming total mileage and behavior were higher,and anoxia survival time were longer in normal group.CGP groups with different dose and western medicine group could prolong the 3 minutes swimming total mileage and anoxia survival time in mice with benzene poisoning（P〈0.05,P〈0.01）.Compared with western medicine group,the 3 minutes swimming total mileage,vertical and horizontal scores were higher in CGP groups with three different dose,anoxia survival time were longer in CGP groups with moderate and low dose（P〈0.05）.Conclusion：CGP may improve stress ability of mice with benzene poisoning merged depression and relieve its depression degree.