摘要
Techniques for detecting glucose are developing at a breathtaking speed because diabetes mellitus can cause many serious complications, such as blindness, high blood pressure heart disease and kidney failure. Herein, water sol- uble NaYF4：Eu^3＋@Ag core-shell nanocrystals for glucose de- tection with lower detection limit have been successfully de- veloped, using NaYF4：Eu^3＋ cores as the energy donors and Ag shells as the efficient quenchers through energy transfer. After immobilization of glucose oxidase （GOx） on the sur- face of NaYF4：Eu^3＋@Ag core-shell nanocrystals, the Ag shells can be decomposed in the presence of glucose, accompanied by down-shifting luminescence recovery. The limit of detec- tion of NaYF4：Eu^3＋@Ag was 0.12 μmol L^-1. Therefore, the NaYF4：Eu^3＋@Ag can be easily extended to the detection of a variety of H2O2-involved analytes.
糖尿病可引起许多严重的并发症,例如失明,血压心脏病和肾衰竭等.因此,葡萄糖检测技术正在以惊人的速度发展.本文合成了水溶性的NaYF_4:Eu^(3+)@Ag核-壳纳米晶体,通过能量转移,Ag壳层可以有效吸收NaYF_4:Eu^(3+)的能量,导致Eu^(3+)离子荧光淬灭.在NaYF_4:Eu^(3+)@Ag核-壳纳米晶体的表面上固定葡萄糖氧化酶(GOx)后,通过加入一定量的葡萄糖,Ag纳米粒子可以分解成Ag^+,并伴随着Eu^(3+)的荧光恢复.结果表明,该葡萄糖检测方法具有非常低的检测限(0.12μmolL^(-1)).
基金
supported by the National Natural Science Foundation of China(21471050 and 21501052)
the China Postdoctoral Science Foundation(2015M570304)
the Postdoctoral Science Foundation of Heilongjiang Province(LBH-TZ06019)
the Natural Science Foundation of Heilongjiang Province(ZD201301d)
the Science Foundation for Excellent Youth of Harbin City of China (2016RQQXJ099)
