不同生态型芸芥中有机物质对盐胁迫的响应

Response of Organic Substance to Salt Stress in Different Eruca sativa Mill. Ecotypes

为了探明有机渗透调节剂在耐盐性中的作用,本研究以芸芥耐盐生态型和不耐盐生态型为试材,测定了盐胁迫下芸芥叶片可溶性蛋白、脯氨酸、可溶性糖等含量。结果表明:渗透势和可溶性蛋白含量在两种生态型间均无明显差异;叶片渗透势和有机渗透调节剂之间没有呈现一致性的变化趋势;不同盐胁迫下,耐盐生态型较不耐盐生态型含有较多的脯氨酸,而在高盐胁迫下,在耐盐生态型中还检测到了高浓度的可溶性糖和游离氨基酸。随着盐浓度的升高,耐盐生态型中可溶性蛋白的总量缓慢增加,但当盐浓度大于200 mmol/L时,可溶性蛋白的含量下降。这些结果初步证明,脯氨酸、可溶性糖和游离氨基酸在盐胁迫应答中起着关键作用,可作为选择耐盐芸芥的标准。

In order to study the effect of organic osmolyte in salt stress, salt-tolerant and non-tolerant ecotypes of Eruca sativa Mill. were used as materials, the content of soluble protein, proline, soluble sugar in leaves were measured. The results showed that osmotic potential and soluble protein content had no significant difference between the two ecotypes, and conforming variation trend was not found between leaf osmotic potential and organic osmolyte. Under different salt stresses, the salt＇tolerant plants had higher proline content than that of non-tolerant plants, and soluble sugar and free amino acid of high concentration were found in salttolerant plants under high salt stress. The content of soluble protein increased slowly in salt-tolerant plants with the increase of salt concentration, and the content of soluble protein decreased when the salt concentration 〉200 mmol/L. The results indicated that proline, soluble sugar and free amino acid played important roles in the responses to salt stress, and could be the standard to select salt-tolerant Eruca sativa.