酸水解-全自动氨基酸分析仪测定方格星虫中氨基酸

Determination of amino acids from Sipunculs nudus by acid hydrolysis-automatic amino acid analyzer

目的:优化测定方格星虫氨基酸的实验条件。方法:研究以酸水解-全自动氨基酸分析仪分析法对海南东方方格星虫中18种氨基酸含量进行分析测定,为确保该实验结果的有效性与可行性,辅以线性关系,加标回收率实验,精密度实验,方法检出限的计算分析。结果:实验发现方格星虫样品中含有18种氨基酸,其中,鲜方格星虫中天门冬氨酸、精氨酸含量较高,分别为2.02%、1.26%,胱氨酸、色氨酸含量最低,分别为0.21%。方格星虫干中谷氨酸、精氨酸含量最高,分别为11.89%、7.94%,而胱氨酸、色氨酸含量最低,分别为0.45%、1.07%;结论:实验方案具备可操作性及数据有效性,仪器的精密度高,相对标准偏差在0.21%以下,检出限低,最低达到0.00000001 ng/kg,线性范围宽,且相关系数在0.9999以上,操作简便,结果令人满意。

Objective： To optimize determination experimental conditions of amino acids from Sipunculs nudus. Method： The author determined the contents of 18 kinds of amino acid by acid hydrolysis-automatic amino acid analyzer with calculation of regression equations and detection limit, experiment of precision and standard recovery, in order to ensure the effectiveness and feasibility of the experimental results.Results：It contains 18 amino acids.In fresh worm the contents of aspartic acid （ 1.26% ） and arginine （ 2.02% ） are the higher, and the contents of cystine （ 0.21% ） and tryptophan （ 0.21% ） are the lowest. In dry worm the contents of glutamic acid （11.89%）and arginine （7.94%）are the highest, and the contents of cystine （0.45%）and tryptophan （1.07%）are the lowest.Conclusion：The experiment scheme has good effect, simple operation, strong operability and valid data,owing to its instrument of high precision and relative standard deviation below 0.21%, low detection limit and the minimum is 0.00000001 mg/kg,wide linear range and the correlation coefficient over 0.9999, so the result is satisfactory.