一氧化氮、血管内皮生长因子与胶质瘤血管形成的关系

Relationship between nitric oxide and vascular endothelial growth factor and glioma angiogenesis

目的 探讨一氧化氮（NO）和血管内皮生长因子（VEGF）在脑胶质瘤血管形成中的作用。方法 采用免疫组化法检测40例不同级别胶质瘤标本中的诱导型NO合酶（iNOS）、VEGF和Ⅷ因子相关抗原（FⅧRAg）的表达,分析其相互关系。结果①对照组无iNOS表达,胶质瘤组iNOS阳性表达率为62.5％,二者有显著性差异（P<0.001）;②iNOS阴性组微血管密度为（22.40±12.62）个·视野-1,iNOS阳性组为（36.90±22.21）个·视野-1,两者间有显著性差异（P<0.05）;③胶质瘤VEGF阳性表达率为65.0％,明显高于对照组（P<0.01）;④VEGF阳性组微血管密度为（38.09±21.69）个·视野-1,阴性组为（19.19±9.05）个·视野-1,两者间差异显著（P<0.01）;⑤iNOS阳性组中VEGF阳性细胞数明显高于iNOS阴性组（P<0.01）。结论 NO和VEGF参与了胶质瘤的血管形成及肿瘤生长,其具有重要的临床和病理学价值。

Objective To investigate the roles of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in glioma angiogenesis. Methods The expressions of inducible nitric oxide synthase (iNOS), VEGF and factorⅧrelated antigen (ⅧRAg) were evaluated by immunohistochemical technique, and their mutual relationships were analyzed in 40 cases of glioma. Results ①The positive expression rate of iNOS was 62.5% in glioma group, while no expression in control group, there was a significant difference between them (P<0.001). ②The microvascular density (MVD) was (22.40 ± 12.62) per field in iNOS negative group and (36.90±22.21) per field in iNOS positive group, there was a significant difference between them (P < 0. 05 ). ③The positive expression rate of VEGF was 65. 0% in glioma group, which was higher than that of the control group (P < 0.01). ④The MVD was (38.09 ±21. 69) per field in VEGF positive group and (19. 19 ±9.05) per field in VEGF negative group, the former was obviously higher than the latter (P<0.01).⑤The VEGF positive cells in iNOS positive samples were more than those in iNOS negative ones (P < 0.01). Conclusion NO and VEGF play important roles in angiogenesis and growth of gliomas, which having important clinical and pathological values.