MLH3基因C2531T多态性与云南地区女性不孕的相关性研究

Correlative analysis of MLH3 gene C2531T polymorphism and female infertility in Yunnan province

目的:研究MLH3基因的C2531T位点多态性和云南地区女性不孕的相关性。方法:收集云南地区140例不孕症女性患者(不孕组)和403例正常育龄期已生育妇女(正常对照组)的血液样本。通过PCR扩增测序方法检测MLH3基因的C2531T多态性。结果:正常对照组MLH3基因的C2531T多态性位点等位基因C和T的频率分别为83.9%和16.1%,不孕组分别为85.0%和15.0%,两组比较差异无统计学意义(P>0.05)。CC、CT和TT基因型在不孕组和正常对照组中分别为70.7%vs 73.2%、26.4%vs 23.6%和2.9%vs 3.2%,两组各基因型频率比较差异均无统计学意义(均P>0.05)。结论:MLH3基因C2531T多态性位点与云南地区女性人群不孕风险不具有相关性。

Objective. To investigate the association between MLH3 gene C2531T single nucleotide polymorphism （SNP） and female infertility in Yunnan province. Methods. 140 cases of infertility women （infertility group, n = 140） and 403 cases of normal child-bearing women （normal control group, n =403） were enrolled in this study. The MLH3 C2531T polymorphism was detected by PCR-sequencing method. Results. The frequencies of MLH3 2531C and T alleles were respectively 83.9% and 16.1% among infertile patients, and were 85.0% and 15.0% among normal controls. The CC, CT and TT genotype frequency of MLH3 C2531T were respectively 70.7%, 26.4% and 2.9% in the infertility patients, and were 73.2%, 23.6% and 3.2% in the normal controls. There was no significant difference in the frequency of genotypes between the two groups （ P 〉0.05）. Conclusion; Genotype frequency of MLH3 C2531T was not associated with the development of infertility women in Yunnan province of China.