根施不同酚酸类物质对东北山樱桃叶绿素荧光特性的影响

Effects of Different Phenolic Acids on Chlorophyll Fluorescence Characteristics of Northeast Hill Cherry

以东北山樱桃(Cerasus sachalinensis Kom.)实生幼苗为试材,在其根际土壤中分别施入不同浓度的对羟基苯甲酸(0.1、1.0、10.0mmol·L^(-1))、香豆素(0.1、1.0、10.0mmol·L^(-1))和对羟基苯甲酸+香豆素二者混合溶液(0.1、1.0、10.0mmol·L^(-1)),利用植物效率仪Hansatch PEA,采用叶绿素荧光诱导动力学理论和JIP-test数据分析方法,研究了根施不同酚酸类物质对东北山樱桃幼苗叶绿素荧光特性的影响。结果表明:不同种类、不同浓度酚酸物质对东北山樱桃幼苗的叶绿素荧光特性产生影响,但作用效果存在差异。其中,不同浓度香豆素(B)和对羟基苯甲酸+香豆素(AB)处理所得结果相似,呈"低促高抑"趋势,即0.1B(AB)>1.0B(AB)>CK>10.0B(AB);而对羟基苯甲酸处理结果则呈现出抑制的趋势,且随浓度增加抑制程度减弱,即CK>1.0A>10.0A>0.1A。PSⅡ有活性反应中心数目在根施酚酸处理后的变化差异极显著。可见,酚酸类物质可能通过改变东北山樱桃幼苗叶片中PSⅡ有活性反应中心数目进而对其光合作用产生影响。

The effects of phenolic acids on fast chlorophyll fluorescence transient and there parameters about the（Cerasus sachalinensis Kom.）were studied via plant efficiency analyzer from Hansatch PEA.In the rhizosphere soil of the northeast hill cherry seedlings,the different concentrations of the hydroxy benzoic acid（0.1,1.0,10.0mmol·L^-1）,coumarin（0.1,1.0,10.0 mmol·L^-1）,hydroxy benzoic acid with coumarin（0.1,1.0,10.0mmol·L^-1）were applied into respectively.The results showed that there were different effects on chlorophyll fluorescence characteristics by different species and different concentrations of phenolic acid.Different concentration treatments of coumarin（B）had similar results as p-hydroxybenzoic acid＋coumarin（AB）,present tendency of promotion in low concentration and inhibition in high concentration that was 0.1B〉（AB）1.0B〉AB〉CK10B（AB）.But the results of effects of p-hydroxybenzoic was inhibition,and the inhibition weakened with the increase of concentration,CK〉1.0A〉10A〉0.1A.In addition,the experimental data showed that the number of active reaction centers of PSⅡchanged significantly after treated by phenolic acids.So,phenolic acids might change the photosynthesis of northeast hill cherry by changing the number of active reaction centers of PSⅡ.