不同栽培基质对霍山米斛总多糖含量的影响

Effect of Different Cultivation Soil on Content of Dendrobium huoshanense Total Polysaccharide

以霍山米斛为试材,于采摘期采摘不同栽培基质培养的霍山米斛,采用苯酚-硫酸显色法测定总多糖含量,探究不同栽培基质对霍山米斛总多糖含量的影响。结果表明:不同栽培基质霍山米斛总多糖含量为S1(松树锯末+石子,低矮大棚)>S3(松树皮+石子,低矮大棚)>S4(椰子壳+石子,低矮大棚)>S2(石子,低矮大棚)>S5(石子,仿野生)。统计学分析表明,不同基质栽培的霍山米斛总多糖含量的差异存在统计学意义,聚类分析数据显示,5种栽培基质的霍山米斛可以分为4类:S1单独一类、S3单独一类、S4单独一类、S2和S5一类。试验旨为霍山米斛的最佳栽培基质选择和初步判定栽培基质的种类提供依据,以期充分地开发霍山米斛资源。

Taking Dendrobium huoshanense as test material, Dendrobium huoshanense were picked in harvest period which were cultivated in different cultivation soil and the content of total polysaccharide of which were determined by phenol-sulfuric acid method,to explore the effects of different cultivation soil on total polysaccharide content of Dendrobium huoshanense. The results showed that the content of total polysaccharide in different cultivation soil were： S1 （sawdust ＋ gravel, greenhouses） 〉 S3 （ pine bark ＋ gravel, greenhouses ） 〉 S4 （ coconut shell ＋ gravel, greenhouses）〉S2（gravel,greenhouses）〉S5（gravel,bionic wild cultivation）. There were significant differences among the total polysaccharide content Denclrobium huoshanense which cultivated in different cultivation soil as the results of statistical analysis. The cluster analysis data showed that Dendrobium huoshanense which cultivated in different cultivation substrate could he divided into four categories：S1 alone classeS3 a separate elass： S4 a separate classeS2 and S5 a class. This study provided the reference for selecting the best type of cultivation soil of Dendrobium huoshanense and preliminary determining the type cultivation soil, in order to develope Dendrobium huoshanense resources adequately.