ApoE缺陷大鼠动脉粥样硬化模型的构建

The establishment of atherosclerosis model in apolipoprotein E-deficient rats

目的构建大鼠的动脉粥样硬化模型。方法将载脂蛋白E(ApoE)缺陷(ApoE-/-)大鼠共16只,均分为两组作为实验组和对照组,均予高脂饲料喂养12周。实验组在前4周予Alzet渗透泵植入大鼠皮下,内部填充血管紧张素Ⅱ(AngⅡ),以1μg/(kg·min)的速率匀速释放。对照组仅予等量生理盐水处理。第12周麻醉后测体重、取血并处死,检测炎症因子及血脂,取心脏及主动脉组织,切片作HE、油红O、Masson染色。结果实验组大鼠出现动脉粥样硬化斑块,对照组无类似表现,两组之间在其他方面无统计学差异。结论 ApoE-/-大鼠予高脂饮食饲养并予AngⅡ刺激,可成功造成大鼠动脉粥样硬化模型。

Aim To establish a novel rat model of atherosclerosis. Methods 16 ApoE deficient rats（ ApoE- /-Rats） were divided into experimental group and control group on the average,and fed with high fat diet for 12 weeks. The experimental group received Alzet osmotic pump implanted subcutaneously in the first 4 weeks,filled with angiotensinⅡ（ AngⅡ）,at a rate of 1 μg /（ kg·min） uniform release,while the control group was only treated with equivalent saline. At the end of the twelfth week,the experimental animals were tested for body weight and blood collected.The inflammatory factors（ interleukin-1β（ IL-1β）,tumor necrosis factor alpha（ TNF-α）） and blood lipids（ total cholesterol（ TC）, triglyceride（ TG）, high density lipoprotein cholesterol（ HDLC）, low density lipoprotein cholesterol（ LDLC）） were detected. Sacrificed after anesthesia,the heart and aortic full length were harvested for HE,oil-red-O and Masson staining. Results The atherosclerotic plaque was found in the experimental group,while the control group had no similar performance. There was no statistical difference between the two groups in other aspects. Conclusion ApoE deficient rats could be induced atherosclerosis plaque successfully,when were treated with high fat diet and AngⅡ.