T7 cDNA噬菌体展示文库筛选法氏囊活性肽BPP-Ⅱ结合蛋白及鉴定

Screening and identification of the binding proteins with BPP-Ⅱ through T7 cDNA phage display

法氏囊活性五肽BPP-Ⅱ是近期发现的具有免疫调节功能的活性多肽。为了研究法氏囊活性肽对B细胞的作用机制,采用鸡B淋巴细胞cDNA T7噬菌体文库筛选法氏囊五肽BPP-Ⅱ与鸡B相互作用的受体蛋白。三轮筛选后,富集的噬菌体滴度达1010pfu以上。富集噬菌体克隆的序列分析结果表明,与BPP-Ⅱ相互作用的鸡B淋巴细胞cDNA文库中序列与核受体辅阻遏物2同源。结果说明,法氏囊活性肽BPP-Ⅱ可能参与多种细胞过程,从而产生多种生物学效应。

The bursa of Fabricius（ BF） is the acknowledged humoral central immune organ,and plays a key role in B lymphocyte development and differentiation. The bursa-derived peptide BPP-Ⅱ is recently reported a biological active peptide on immune induction. However,the mechanism by which this active molecular plays role in B lymphocyte is little reported. To investigate the mechanism,in this paper,the interacting proteins with BPP-Ⅱ were screened by T7 B cell c DNA phage display library. These results showed that after three screening and sequencing,the titer of enriched phage was up to 1010 pfu,and the interacting gene of BPP-Ⅱ in avian B cell was homologous to nuclear receptor corepressor 2. These results suggested that BPP-Ⅱ participates in various biological functions,which provides some new insight for the biological function and mechanism of BF on immune induction.