L型氨基酸转运体1和血管内皮生长因子在甲状腺乳头状癌中的表达及意义

Expressions of L-type amino acid transporter 1 and vascular endothelial growth factor in papillary thyroid carcinoma and their clinical significance

目的探讨L型氨基酸转运体1(LAT1)和血管内皮生长因子(VEGF)在甲状腺乳头状癌(PTC)中的表达及意义。方法采用免疫组织化学法检测68例PTC和相应癌旁组织中LAT1蛋白及VEGF蛋白的表达,并分析LAT1与PTC临床病理参数的关系及LAT1和VEGF的相关性。结果 LAT1蛋白在PTC组织和相应癌旁组织中的阳性表达率分别为83.8%和8.8%,VEGF蛋白在PTC组织和相应癌旁组织中的阳性表达率分别为88.2%和7.4%,LAT1蛋白和VEGF蛋白在PTC组织中的阳性表达率均高于癌旁组织(P<0.05);LAT1蛋白在PTC组织中的阳性表达与肿瘤临床分期和淋巴结转移相关(P<0.05),与年龄、性别和肿瘤大小无关(P>0.05);PTC组织中LAT1蛋白和VEGF蛋白表达呈正相关(P<0.05)。结论 LAT1可能通过调控VEGF表达而促进PTC的发生与演进,并可能成为PTC治疗的一个新的分子靶点。

Objective To explore the expression of L-type amino acid transporter 1（ LAT1） and vascular endothelial growth factor（ VEGF） in papillary thyroid carcinoma（ PTC） and their clinical significance. Methods The expression of LAT1 and VEGF in 68 cases of PTC and para-carcinoma tissue were detected by immunohistochemisy. The relationship between the expression of LAT1 protein and clincopathological parameters of PTC were analyzed; the correlation between the expression of LAT1 protein and VEGF protein were analyzed. Results The positive expression rate of LAT1 protein in PTC and para-carcinoma tissue was 83. 8% and 8. 8% respectively; the positive expression rate of VEGF protein in PTC and para-carcinoma tissue was 88. 2% and 7. 4 % respectively; the positive expression rate of LAT1 and VEGF protein in PTC tissue was significantly higher than that in para-carcinoma tissue（ P〈0. 05）. The expression of LAT1 protein was related to lymph node metastasis and clinical stage of tumor（ P〈0. 05）,while the expression of LAT1 protein was not related to age,sex and tumor size（ P〉0. 05）. There was positive correlation between the expression of LAT1 protein and VEGF protein in PTC（ P〈0. 05）. Conclusion LAT1 may promote the occurrence and progression of PTC by regulating the expression of VEGF,which may provide a new therapeutic target for the treatment of PTC.