摘要
[目的]探讨姜黄素对结肠癌细胞上皮间质转化(EMT)的作用机制。[方法]通过MTS检测姜黄素对HCT116细胞活性的影响;运用Western-blot、Real-time q PCR检测不同浓度姜黄素对HCT116细胞Wnt相关基因(β-catenin、TCF4)及EMT相关基因(E-cadherin和Vimentin)的表达情况。[结果 ]姜黄素能明显抑制HCT116细胞的增殖活性,在12h、24h和48h的IC50分别是61.98±2.68μmol/L,19.64±1.29μmol/L和17.84±1.25μmol/L。姜黄素能下调HCT116细胞内β-catenin和TCF4的表达水平;同时显著性上调E-cadherin及下调Vimentin表达水平,且呈剂量依赖性。Wnt信号通路活化剂能上调姜黄素处理后细胞内的β-catenin表达和下调E-cadherin表达水平。[结论]姜黄素通过抑制Wnt/β-catenin信号通路,从而抑制肿瘤细胞上皮间质转化。
[Objective] To investigate the mechanism of curcumin on epithelial-mesenchymal transition(EMT) of colon cancer cells. [Methods] The effects of curcumin on the activity of HCT116 cells were detected by MTS. The expression of Wnt-related genes(β-catenin and TCF4) and EMTrelated genes(E-cadherin and Vimentin) were detected by Western-blot and real-time q PCR assay in HCT116 cells. [Results] Curcumin could significantly inhibit the proliferation of HCT116 cells and the IC50 at 12h、24h and 48 h respectively were 61.98 ±2.68μmol/L,19.64 ±1.29μmol/L and17.84 ±1.25μmol/L respectively. Curcumin could also down-regulate the expression of β-catenin and TCF4 in HCT116 cells. At the same time,curcumin could up-regulate the expression of Ecadherin and down-regulate the expression of Vimentin in a dose-dependent manner in HCT116 cells. After treatment with Wnt signaling pathway activator,the expression of β-catenin increased and the expression of E-cadherin decresed in HCT116 cells treated with curcumin. [Conclusion]Curcumin can inhibit the epithelial-mesenchymal transition by inhibiting the Wnt/β-catenin signaling pathway in tumor cells.
作者
陈海滔
徐超
姚庆华
CHEN Hai-tao XU Chao YAO Qing-hua(Second Clinical College of Zhejiang Chinese Medical University ,Hangzhou 310053, China First Clinical College of Zhejiang Chinese Medical University,Hangzhou 310053,China Zhejiang Cancer Hospital,Key Laboratory of Traditional Chinese Medicine Oncology,Zhejiang Cancer Hospital, Hangzhou 310022, China)
出处
《肿瘤学杂志》
CAS
2017年第1期35-39,共5页
Journal of Chinese Oncology
基金
国家自然科学基金(81673813)