一种改良的三血管闭塞小鼠缺血性脑卒中模型的建立

A modified three-vessel occlusion mouse model with ischemic stroke

目的建立一种稳定可靠的小鼠缺血性脑卒中模型。方法 50只雄性C57BL/6J小鼠,随机分为三血管闭塞组(3VO,15 min短暂夹闭双侧颈总动脉合并永久电凝右侧大脑中动脉远端,n=20),线栓对照组(MCAO control,传统的线栓法MCAO模型,n=20),假手术组(sham,暴露三血管但不予以闭塞,n=10)。术后24 h行为学评分后,部分动物以TTC染色计算脑梗死体积(n=10),部分动物用以观察存活率(n=10)。结果术后24 h 3VO组转角实验的偏侧指数显著低于假手术组(-0.7 vs-0.04,P<0.01)。TTC染色发现3VO小鼠脑梗死体积为17.6%±1.6%(n=10);MCAO对照组脑梗死体积为42.6%±15.0%(n=10)。3VO组动物7 d存活率明显高于MCAO组(90%vs 60%,n=10)。结论三血管闭塞法所产生的小鼠缺血性脑卒中模型稳定、可靠,适用于脑缺血研究。

Objective To develop a reliable and reproducible model of ischemic stroke. Methods Male C57 BL /6J mice were randomly divided into three groups： three-vessel occlusion（ 3VO,15-min temporary occlusion of the bilateral common carotid arteries superimposed on a permanent occlusion of the right distal middle cerebral artery,n = 20）,suture MCAO control（ n = 20）,and sham-operated group（ n = 10）. At 24 h functional outcome was assessed using corner test,cerebral ischemic lesion was determined by TTC staining（ n = 10）,and then the surviral rate was measured till day 7（ n = 10）. Results The 3 VO group got significantly lower neurological deficit score as compared to sham-operated group（-0. 7 vs-0. 04,P〈 0. 01）. Infarct volume of mice in 3 VO group was17. 6% ± 1. 6%,while 42. 6% ± 15. 0% in MCAO group（ n = 10）. The 3VO group got a significantly increased7-day survival rate compared to suture MCAO group（ 90% vs 60%,n = 10）. Conclusions The present three-vessel occlusion model is stable and reliable for research on ischemic stroke.