急性髓系白血病患者化疗后微小残留病与多药耐药基因表达相关

Correlation of minimal residual disease and multidrug resistance genes expression in acute myeloid leukemia

目的明确急性髓系白血病(AML)中微小残留病(MRD)与多药耐药基因表达的相关性。方法收集初诊AML患者骨髓52例,用real-time PCR检测患者化疗前ABCB1、ABCC1、ABCC4及ABCG2 4种多药耐药基因的表达,同时用多参数流式细胞术(MFC)检测患者诱导化疗后和化疗3、6和9个月后的MRD表达水平。结果 52例AML患者MRD表达水平与ABCB1(P<0.01)、ABCC1(P<0.01)、ABCC4(P<0.01)和ABCG2(P<0.01)的mRNA表达水平具有明显相关性。持续监测9个月后,MRD再次转阳者ABC基因表达水平显著高于持续阴性者:ABCB1(P<0.05)、ABCC1(P<0.05)、ABCC4(P<0.01)和ABCG2(P<0.01)。结论 MRD表达水平与多药耐药基因表达水平显著正相关。

Objective To explore the correlation of minimal residual disease（ MRD） and ABC genes expression in acute myeloid leukemia（ AML）. Methods 52 de novo AML bone marrow samples were used to detect the expression of ABCB1,ABCC1,ABCC4 and ABCG2 by real-time PCR at diagnosis. Meanwhile,followed up these patients to monitor MRD with MFC at the points of finishing the first induction cause as well as the third,the sixth and the ninth month after chemotherapy. Results The expression level of 4 ABC transporters among three MRD level detected after induction cause are correlated significantly with ABCB1（ P 〈0. 01）,ABCC1（ P 〈0. 01）,ABCC4（ P〈 0. 01） and ABCG2（ P 〈0. 01）. Further more,after nine months follow-up,the patients whose MRD turned into positive again were detected a high ABC transports expression level,comparing with those MRD remained negative for nine months,which was of a statistically significance for ABCB1（ P 〈0. 05）,ABCC1（ P 〈0. 05）,ABCC4（ P〈 0. 01） and ABCG2（ P 〈0. 01）. Conclusions A positive correlation of MRD and ABC transporters level in AML is found.