黄连素对大鼠心肌缺血再灌注所致心肌损伤的保护作用

Protective effect of berberine on myocardium ischemia reperfusion induced-myocardial damage

目的探讨黄连素对缺血再灌注所致心肌损伤的保护作用。方法 60只雄性健康SD大鼠随机分为5组,即对照组、模型组、阳性对照组及黄连素低[75 mg/(kg·d)]、高[150 mg/(kg·d)]剂量组。黄连素连续灌胃给药14 d后,除对照组外,其余各组结扎冠状动脉左前降支建立大鼠心肌缺血再灌注模型,监测心电图,记录心律失常评分;检测血浆中心钠肽(ANP)、脑钠肽(BNP)、心肌肌钙蛋白-I(c Tn-I)浓度;HE染色与Masson染色方法检测心肌机构损伤情况。结果黄连素预防给药可缩短缺血再灌注所致心律失常恢复正常的时间,同时显著抑制血浆中ANP、BNP和c Tn-I水平的上升,改善心肌结构。结论黄连素对缺血再灌注所致的心肌损伤具有保护作用。

Objective To determine the protective effect of berberine on ischemia-reperfusion induced myocardial injury. Methods Sixty healthy male Sprague-Dawley rats were randomly divided into 5 groups： a control group, a model group, a positive control group, a low dose berberine [75 mg/（kg.d）] group and a high dose [150 mg/（kgod）] group. The left anterior descending branch of coronary artery was ligated out to establish the model of myocardial ischemia-reperfusion in rats. The electrocardiogram and the cardiac arrhythmia score were recorded. The plasma levels of atrial natriuretic peptide （ANP）, brain natriuretic peptide （BNP） and cardiac troponin-I （cTn-I） were detected by ELISA. The myocardial injury was detected by HE staining and Masson staining. Results The administration of berberine shortened the recovery time of arrhythmia induced by ischemia-reperfusion, and significantly inhibited the increase in plasma ANP, BNP and cTn-I levels and improved the myocardial structure. Conclusion Berberine has protective effect on myocardial injury induced by ischemia-reperfusion.