层次分析联合Box-Behnken效应面法优选复方首乌藤合剂提取工艺

Optimization of extraction of compound Shouwuteng mixture by analytic hierarchy process and Box-Behnken design response surface methodology

目的优选复方首乌藤合剂的提取工艺。方法以2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、(-)-丁香树脂酚-4-O-β-D-呋喃芹糖基-(1→2)-β-D-吡喃葡萄糖苷、金丝桃苷的提取率以及浸膏得率为综合评价指标,应用层次分析法确定权重系数,选择浸泡时间、溶剂用量和提取时间为考察因素,在此基础上再进行提取次数考察。结果复方首乌藤合剂的最佳提取工艺为浸泡4 h,提取2次,每次3 h,溶剂用量为10倍量。结论该工艺稳定可行,实际值与预测值吻合度高,预测性良好,优选的工艺各成分提取率高,为复方首乌藤合剂的研究提供了实验依据。

Objective To optimize the extraction of compound Shouwuteng mixture. Methods With the extraction rate of 2, 3, 5, 4＇-tetrahydroxystibene-2-O-β-D-glucoside, syimgaersinol-4-O-β-D-glucopyranoside, hyperin and dry extract yield, analytic hierarchy process was adopted to determine the weight coefficient. The soaking time, and the ratio of water and extraction time were investigated by Box-Behnken design response surface methodology, and on the basis of this, the extraction times was studied. Results The optimal extraction technology was as follows： soaking for 4 h, extraction twice and 3 h each time, with 10-folds of water. Conclusion The optimized extraction technology is stable and feasible with high predictability, and it provides an experimental basis for the research of compound Shouwuteng mixture.