重度子痫前期患糊S6基因834＋7G／A和＋1332C／T多态性的研究

Association study between 834q-7G/A and ＋ 1332C/T polymorphisms in the growth arrest specific 6 gene and risk of severe preeclampsia in Chinese population

目的探讨生长停滞特异基因6（growth arrest specific gene6，GAS6）多态性是否与重度子痫前期发病有关联。方法采用聚合酶链反应一限制性片段长度多态性分析法对成都地区167例重度子痫前期患者和312名健康孕妇GAS6基因834＋7G／A和＋1332C／T多态性进行分析。结果GAS6基因＋1332C／T位点C、T等位基因的频率在重度子痫前期组为85．63％、14．37％，在正常孕妇组为78．04％、21．96％。＋1332C／T位点基因型和等位基因频率在重度子痫前期组和正常妊娠对照组之间差异具有统计学意义（均P〈0．05），CT＋TT基因型携带者在患者组的频率显著低于对照组（28．14％VS39．42％），发生重度子痫前期的风险降低（OR=0．602，95o．4CI：0．401～0．904，x^2=6．045，P=0．014）。834＋7G／A位点G、A等位基因的频率在重度子痫前期组为72．75％、27．25％，在正常孕妇组为74．36％、25．64％。两组之间基因型和等位基因频率差异无统计学意义（均P〉0．05）。此外，重度子痫前期组和正常妊娠对照组GAS6基因两位点基因型对血压水平未见显著影响。结论中国成都地区汉族人GAS6基因＋1332T等位基因与重度子痫前期发病的风险降低可能存在关联，但未见834＋7G／A位点与重度子痫前期的发生有关。

Objective To investigate the relationship between polymorphisms of the growth arrest specific 6 （GAS6） gene and severe preeclampsia in a South West Han Chinese population. Methods Blood samples from 167 patients with severe preeclampsia and 312 normal pregnant women as controls from Han Chinese in Chengdu area were analyzed by polymerase chain reaction-restriction fragment length polymorphisms. Results C and T allele frequencies for ＋1332C/T site were 85.63% and 14.37% in the patient group, respectively, and 78.04% and 21.96% in control group, respectively. The TT genotype and variant T allelic frequencies of the ＋1332C/T polymorphism were significantly lower in patients with severe preeclampsia than in the control group （both P 〈 0. 05）, and the odds ratio for the risk of severe preeclampsia was 0. 602 （95%CI： 0. 401-0. 904） in carriers for the variant T allele （x^2= 6. 045, P= 0. 014）. G and A allele frequencies for 834＋ 7G/A site were 72. 75% and 27. 25% in case group, respectively, and 74.36% and 25.64% in control group, respectively. The genotype and allele frequencies of the 834 ＋ 7G/A polymorphism in patients with severe preeclampsia and controls showed no significant differences （both P〉0.05）. In addition, there was no significant association between the polymorphisms and blood pressure levels in the patient or control groups. Conclusion The variant GAS6 ＋1332 T allele is associated with a decreased risk for severe preeclampsia in a South West Han Chinese population. On the other hand, the 834＋TG/A polymorphism has no effect on the severe preeclampsia.