厦门地区汉族人群15个STR基因座遗传多态性研究

Analysis of genetic polymorphisms of 15 STR loci among ethnic Hans from Xiamen

目的调查福建厦门地区汉族群体15个短串联重复序列（short tandem repeat，STR）基因座多态性参数，同时评估AmpFLSTR Identifiler^TM体系应用于本地区汉族人群进行法医学个体识别及亲子鉴定的价值。方法应用AmpFLSTR Identifiler^TM体系荧光标记复合扩增系统，检测400名福建厦门地区汉族无关个体15个STR基因座的多态性，统计计算群体遗传学参数。结果15个STR基因座的基因型分布均符合Hardy-Weinberg平衡（P〉0．05）。15个STR遗传标记均具有高度多态性，杂合度0．580～0．868，匹配概率0．036～0．148，个体识别力0．798～0．967，多态性信息含量0．560～0．850，非父排除率0．268-0．730。结论15个STR基因座在福建厦门地区有较高的多态性。通过检测等位基因频率，为福建厦门地区人群法医学亲权鉴定和个人识别等位基因多样性提供了客观依据。

Objective To assess the value of 15 short tandem repeat （STR） loci selected by an AmpFLSTR IdentifilerTM system for personal identification and paternity testing among ethnic Hans from Xiamen, Fujian. Methods For 400 unrelated individuals, allelic frequencies for the 15 STR loci from the AmpFLSTR IdentifilerTM kit were determined. Population genetics parameters for forensic usage were calculated. Results No deviation of the observed allele frequency from Hardy-Weinberg equilibrium expectations was found by Chi-square test （P〉 0. 05）. All of the 15 loci were highly polymorphic. Observed heterozygosity has varied between 0. 580 and 0. 868. Matching probability was between 0. 036 and 0. 148. Power of discrimination was between 0. 798 and 0. 967. Polymorphic information content was between 0. 560 and 0. 850. And power of exclusion was between 0. 268 and 0. 730. Conclusion All of the 15 loci selected by the AmpFLSTR Identifiler^TM system are highly polymorphic among ethnic Hans from Xiamen. By determining the alleles and allelic frequencies, data for genetic polymorphisms usable for paternity testing and personal identification for local population were obtained.