草莓采后灰霉病生防菌Burkholderia contaminans培养基优化分析

Medium Optimization Analysis of Biocontrol Bacteria Burkholderia contaminans B-1 Against Fruit Postharvest Grey Mould

旨在研究洋葱伯克霍尔德菌Burkholderia contaminans在摇瓶条件下的生物量最大培养基配方。试验采用多因素二次通用旋转组合设计的方法,通过对不同碳源、氮源和无机盐种类和浓度对B.contaminans生长的影响,确定拮抗菌浓度最大的培养基配方。筛选出的最佳培养基配方为:麦芽浸粉2.11%、酵母提取物1.90%、磷酸二氢钾0.05%,硫酸锰0.05%。在该培养条件下,伯克氏菌Burkholderia contaminans B-1生物量达到最大值,且经过进一步验证,其优化效果较好。拮抗菌株B.contaminans培养条件的优化分析为该菌高产发酵工艺的建立奠定了基础,促进了该菌株在果蔬采后生物防治的应用与产业化开发。

This objective was to discover the culture medium formulation of Burkholderia contaminans with the biggest biomass yield under shake flask conditions. In this experiment, we used the multi-factor quadratic general rotary unitized design method to study the effects of varied carbon sources, nitrogen sources and inorganic salt types ＆ concentrations on strain growth, and finally determined the proper formulation. The optimal medium formulation was as follows： malt powder 2.11%, yeast extract 1.90%, KH2PO4 0.05% and MnSO4 0.05%. It had been proved that the biomass of B. contaminans reached the maximum under this condition and showed better optimization effects by further detection. This optimization analysis will pave the way for establishing high yield fermentation process of B. contaminans, and facilitate its application in after- harvest biocontrol of fruits and vegetables, and industrialized development as well.