玉米弯孢叶斑病菌Fus3/Kss1-MAPK级联途径的相关基因鉴定与结构特征分析

Identification and Structure Characteristics of Related Genes of Fus3/Kss1-MAPK Pathway from Curvularia lunata

植物病原真菌Fus3/Kss1-MAPK级联途径在调控病原菌附着胞形成和致病性等方面起重要作用。为了明确Fus3/Kss1-MAPK级联途径在玉米弯孢叶斑病菌中的调控作用,笔者采用生物信息学方法,对玉米弯孢叶斑病菌全基因组中的Fus3/Kss1-MAPK级联途径的相关基因进行鉴定并对其分子结构特征分析。结果表明:从玉米弯孢叶斑病菌中鉴定出Fus3/Kss1-MAPK级联途径中3个蛋白激酶基因Clf、Map2k、Clk1和一个锚定蛋白基因Cl Ste50。蛋白序列分析以及进化树构建等表明它们分别与来源其他植物病原真菌的Fus3/Kss1-MAPK途径中的激酶蛋白和锚定蛋白具有相似的结构特征和保守结构域,有较高同源性和较近的亲缘进化关系。玉米弯孢叶斑病菌Fus3/Kss1-MAPK途径的相关蛋白激酶Clk1、Map2k、Clf和锚定蛋白Clste50与其他真菌Fus3/Kss1-MAPK途径的相关蛋白结构相似,推断有相似的功能。

The cascade pathway of Fus3/Kssl-MAPK in plant pathogenic fungi plays an important role in regulating appressorium development and pathogenicity. In order to investigate the role of Fus3/Kssl-MAPK cascade pathway in ＇Curvularia lunata＇, we used bioinformatics methods to identify the related gene of the Fus3/Kssl-MAPK cascade pathway from ＇ Curvularia lunata＇ in genome-wide and analyzed the molecular structure characteristics of Fus3/Kssl-MAPK related gene. The result showed that three protein kinases genes of Clf, Map2K, Clkl and an ankyrin gene ClsteSO were identified based on genome of ＇ Curvularia lunata＇. The protein sequence analysis and phylogenetic tree construction showed that Clf, Map2k, Clk1 and Clste50 in ＇ Curvularia lunata＇ all contained similar structural features and conservative domains with those of other plant pathogenic fungi, and had high homology and close phylogenetic relationships. The related protein kinases genes Clf, Map2K, Clkl and an ankyrin gene ClsteSO of the Fus3/Kss1-MAPK cascade pathway from ＇ Curvularia lunata＇ were similar to the protein structures with other fungi. Those results may indicate that they have similar function in appressorium development and pathogenicity.