电脉冲刺激的骨骼肌细胞条件培养基逆转内皮细胞胰岛素抵抗和功能障碍

Conditional medium from electric pulse-stimulated C2C12 skeletal muscle cells reverses insulin resistance and endothelial dysfunction in endothelial cells

目的:探讨电脉冲刺激的C2C12肌管细胞条件培养基对内皮细胞的影响。方法:提取常氧和缺氧培养的3T3-L1脂肪细胞上清液为条件培养基CM-N和CM-H,电脉冲刺激的C2C12肌管细胞上清液为条件培养基CM-EPS,单独或按比例混合分别孵育内皮细胞16 h。Western blot检测Akt、eNOS、IKK和NF-кB的磷酸化。结果:CM-H降低内皮细胞Akt和e NOS的磷酸化,并升高IKK和NF-кB的磷酸化。CM-EPS逆转此作用。结论:CM-EPS逆转CM-H造成的内皮细胞胰岛素抵抗并改善内皮功能。

Objective： To explore the effects of conditional medium from electric pulse-stimulated C2C12 myotube on endothelial insulin resistance and dysfunction. Methods： The supernatant of normoxic and hypoxic 3T3-L1 adipocytes were collected as conditional medium CM-N and CM-H, respectively. The supernatant of pulsed electrical-stimulated C2C 12 myotube was collected as conditional medium CM- EPS. Endothelial cells were incubated with CM-N, CM-H and CM-EPS for 16 hours, respectively. The phosphorylation of Akt, eNOS, IKK and NF-KB in endothelial cells were analyzed by Western blot. Results： The phosphorylation of Akt and eNOS were increased significantly, and the phosphorylation of IKK and NF-KB were reduced under CM-H treatment, which were reversed by adding CM-EPS. Conclusion： CM-EPS can reverse insulin resistance in endothelial cells and improve endothelial dysfunction induced by CM-H.