丹参酮ⅡA对EAE小鼠临床表现及外周Treg细胞的影响

Effects of Tanshinon ⅡA on the clinical manifestation and peripheral Treg of EAE mice

目的:探讨丹参酮ⅡA对实验性自身免疫性脑脊髓炎(EAE)小鼠临床表现以及外周调节性T细胞(Treg)的影响。方法:用MOG_(35-55)混合完全弗式佐剂免疫C57BL/6雌性小鼠,免疫后随机分为两组,发病后治疗组每日腹腔注射丹参酮ⅡA,对照组注射同体积的磷酸盐缓冲液,观察小鼠体质量及临床评分的变化,第26天处死小鼠后进行免疫学实验和细胞计数,分析两组小鼠中枢神经系统中炎性细胞浸润和髓鞘脱失情况以及中枢和外周淋巴细胞亚群的变化。结果:丹参酮ⅡA治疗组与对照组相比,小鼠的临床评分下降,中枢神经系统中炎性细胞浸润和髓鞘脱失面积均减少,细胞计数显示外周分泌Foxp3的CD4+T细胞占CD4+T细胞群的14.1%,较对照组明显升高(P<0.05)。结论:丹参酮ⅡA可以显著减少EAE中枢神经系统中炎性细胞的浸润和髓鞘脱失,增加外周调节性T细胞的分泌,改善实验动物的临床表现。

Objective： To explore the effects of Tanshinon Ⅱ A （Tan ⅡA）on the clinical manifestation and peripheral regulatory T cells （Treg） of experimental autoimmune encephalomyeiltis （EAE） mice. Methods： C57BL/6 mices were divided into two groups randomly after EAE model was established by MOG35-55. In the treatment group, Tan Ⅱ A was injected intraperitoneally daily while control group was injected with the same dose of PBS after onset. Then the clinical score of mices was monitored. The inflammation, demyelination and the changes of lymphocyte subsets were analyzed in the two groups after all the miees were put to death on the 26th day, Results： The results showed that compared with the control group, mice in Tan ⅡA group had significantly lower clinical scores, less aera of inflammatory infiltration and demyelination. And the ratio of Foxp3 secreted by CD4＋T cells was 14.1%, which was increased significantly （P〈0.05）. Conclusion： Tan Ⅱ A can reduce the demyelination and the number of inflammatory cells in EAE while increase the regulatory T cells. Tan ⅡA can also improve the manifestation of the mices.