秋水仙素诱导黑喉石斛多倍体研究

Study on Polyploid of Dendrobium ochreatum Induced by Colchicine

以黑喉石斛原球茎为材料,探讨了秋水仙素不同处理对黑喉石斛多倍体诱导的影响.流式细胞仪测定结果表明,适宜四倍体诱导的秋水仙素质量分数在0.01%~0.03%之间,适宜八倍体诱导的秋水仙素质量分数在0.05%~0.10%之间,处理时间均为2~3 d;获得的多倍体在形态、气孔和染色体数目上有明显变化;八倍体与四倍体相比,发育更加迟缓,多表现为整株分叉.

Effects of the various colchicine treatments on polyploidy induction of Den.ochreatum were carried out in the current work.The results measured by flow cytometry showed that the most efficient condition of Den.Ochreatum for inducing tetraploids seemed to be treated with 0.01%-0.03% colchicine and octoploids with 0.05%-0.1% colchicine all for 2-3 days.The polyploidies were different from normal diploids in morphology,stoma and chromosome numbers.Compared with tetraploid,octoploid developed more slowly,and more showed bifurcation.