猪源沙门氏菌的耐药性检测与分析

Antimicrobial Susceptibility Testing of Salmonella in Fresh Pork

采集辽宁省大连市鲜猪肉78份,选择性培养基分离沙门氏菌,划线纯化后,通过inv A序列对菌株进行鉴定,采用纸片琼脂扩散法(K-B法)检测沙门氏菌对12种抗生素的耐药性。结果共得到15株沙门氏菌,检出率为19.23%。沙门氏菌对各种抗生素的耐药率在0%~73.3%,其中对硫酸庆大霉素的耐药率最高,为73.3%;对其他抗菌药物的耐药率依次是盐酸诺氟沙星(60%)、氟苯尼考、盐酸环丙沙星(53.3%)>阿莫西林(13.3%)>头孢噻吩、头孢噻肟、氨苄西林(6.7%)>头孢曲松、头孢他啶、氨曲南、亚胺培南(0%),共产生10种耐药谱。由此表明,目前大连市开发区的超市和农贸市场受沙门氏菌污染率较高,并且沙门氏菌对各类抗生素表现出了多重耐药谱,应该给予足够的重视。

Seventy -eight pork samples were collected in Dalian city Liaoning province. Salmo- nella were isolated using selective medium. The isolates were identified by invA sequence analy- sis. The drug resistance of Salmonella to 12 kinds of antibiotics was detected by the standard disk diffusion method （K- B）. The results show that 15 Salmonella were obtained with detec- tion frequency of 19.23%. The drug resistance rate of Salmonella to antibiotics was 0 to 73.3%, 73.3% of 15 Salmonella isolates were resistant to GEN. The resistance frequency to other antibiotics decreased in the following order： NIA （60%） 〉 FLO, CH （53.3%） 〉 AMO （ 13.3% ） 〉 KF,CTX,AMP（6.7% ） 〉 CRO,CAE,ATM,IPM（0% ）. There were 10 resistance profiles. City supermarkets and farmers＇ markets in Dalian Development Zone is affected by higher rate of Salmonella contamination, and the Salmonella showed multiple resistance to anti- biotics. The multi -drug resistant spectrum should be given sufficient attention.