羧基化半抗原直接包被酶联免疫吸附实验法测定水中残留阿特拉津

Carboxylated hapten coated directly coated ELISA for the detection of atrazine residue in water

目的探讨新型羧基化半抗原直接包被酶联免疫吸附实验(ELISA)法测定水中残留阿特拉津的可行性。方法使用烷基化试剂3-氨基丙基三乙氧基甲硅烷(APTES)在聚苯乙烯表面构建活性的伯胺基,羧基化阿特拉津经活化后进攻伯胺基形成稳定的酰胺键从而完成羧基化半抗原直接偶联。羧基化半抗原直接包被ELISA法测定水中阿特拉津残留。结果该方法检出限为0.68 ng/mL。该抗体与西玛津交叉反应率为24%,其他类似物交叉反应率均小于0.1%,特异性较高。在实际样本分析中具有较高的准确度(添加回收率94.0%~112.0%)和稳定性(相对标准偏差2.72%~3.53%)。结论该方法用于检测水中残留阿特拉津,方法简便,结果可靠。

Objective To develop the carboxylated hapten coated enzyme-linked immunosorbent assay（ ELISA） for the detection of atrazine in drinking water. Methods Polystyrene surface was modified by 3-aminopropyltriethoxysilane（ APTES） to produce amino groups for the directly immobilization of carboxylated atrazine on the surface of microtiter plates. Results The carboxylated hapten coated directly coated ELISA showed higher sensitivity（ 0. 68 ng / m L） and higher specificity. In real sample analysis, the recoveries were ranged from 94. 0% to 112. 0%,and the relative standard deviation was 2. 72%- 3. 53%. Conclusion The method is simple,reliable,and can be used to detect atrazine in drinking water.