脉冲辐射与表面活性剂处理对甘草种子发芽的影响

Effect of Pulse Radiation and Surfactant Treatment on Seed Germination of Glycyrrhiza

目的:通过比较脉冲辐射、表面活性剂、氢氧化钠及浓硫酸处理对甘草种子发芽影响,探究找出甘草种子预处理的最佳方式。方法:以浓硫酸处理为对照组,分别使用RS-1数字编码脉冲处理仪的高、低档位辐射甘草种子,进行发芽率测定实验。以浓硫酸处理、氢氧化钠处理和去离子水处理的甘草种子为对照。使用SDS十二烷基硫酸钠,3-磺丙基十二烷基甜菜碱,苯扎氯铵,吐温-80四种不同种类表面活性剂处理甘草种子,进行发芽率测定实验。结果:脉冲处理的3种甘草种子,种子发芽率均高于自然发芽率。特别是利用高档位脉冲处理的不同甘草种子发芽率和发芽势与浓硫酸处理组无显著性差异。表面活性剂处理的甘草种子发芽率普遍高于自然发芽率,但发芽率显著低于浓硫酸处理组,除苯扎氯铵组外,其余各表面活性剂处理组发芽率与去离子水处理组无显著差异。结论:处理甘草种子时,可以考虑通过使用高档位脉冲辐射处理作为替代浓硫酸处理的一种方式。这样不仅能减少浓硫酸导致的环境污染,还能降低处理成本。而用表面活性剂SDS十二烷基硫酸钠,3-磺丙基十二烷基甜菜碱,苯扎氯铵,吐温-80处理组提高甘草种子发芽率的效果不显著。

Objective： By contrast the influences of pulse radiation treatment, surfactant treatment, sodium hydroxide treatment and sulfuric acid treatment on glycyrrhiza seed germination, to find the best way to improve the rate of glycyrrhiza seed germination. Methods： Specific methods is to use RS-1 digital encoder pulse radiate glycyrrhiza seed, which is divided into high level and low level, set the sulfuric acid treatment as the control group. Using SDS sodium dodecyl sulfate, dodecyl 3-sulfopropyl betaine, benzalkonium chloride, Tween-80 four different types of surfactant treatment of glycyrrhiza seeds and the sulfuric acid treatment, sodium hydroxide treatment and deionized water treatment of glycyrrhiza seeds as the control group, to do the measurement experiment of germination rate. Results： The results showed that the three kinds of glycyrrhiza seeds processed by the pulse radiation treatment, their germination rate are higher than the natural rate of germination. Especially the glycyrrhiza gets higher rate of seed germination after processed by high-grade pulse, which is almost the same as the results after processed by sulfuric acid. Seeds germination rate of surfactant treatment is generally higher than the natural germination rate, but obviously lower than the seed of sulfuric acid treatment. Except the results of benzalkonium chloride treatment, other surfactant treatments＂ results do not have disparity between the result of deionized water treatment. Conclusion： Therefore, the glycyrrhiza may can high level pulse radiate seed method as an instead of sulfuric acid method for processing seeds method. This method not only reduces sulfitric acid＇s pollution of the environment, but also can reduce processing costs. The SDS surfactant sodium dodecyl sulfate, sodium dodecyl 3-sulfopropyl betaine, benzalkonium chloride, Tween-80 treatment increased germination effects are not significant.