复方楂金颗粒剂对非酒精性脂肪性肝炎大鼠PPAR-α影响的实验研究

Experimental Study on Effects of Fufang Zhajin Granule(复方楂金颗粒)on PPAR-α Expression of Rats with Non-alcoholic Fatty Liver Disease

目的:探讨复方楂金颗粒剂(FZJG)对非酒精性脂肪性肝炎(NASH)大鼠过氧化物酶体增殖物激活受体α(PPAR-α)表达的影响。方法:雄性SD大鼠随机分为6组,正常组(正常饮食),模型组、阳性对照组和复方楂金颗粒高中低剂量组给予高脂饲料喂养10周诱发脂肪性肝炎后分别以生理盐水、易善复、复方楂金颗粒剂高、中、低剂量灌胃,每天1次,共10周。处死大鼠,分别进行如下检测:1计算肝指数;2HE染色,光镜下观察各组大鼠肝组织的病理改变;3免疫组织化学染色和RT-PCR法检测大鼠肝组织PPAR-α蛋白和mRNA的表达。结果:模型组大鼠肝指数较正常组明显增加(P<0.01);FZJG中剂量组、低剂量组大鼠的肝指数低于模型组,有统计学意义(P<0.05,P<0.01)。模型组NAFLD活动度积分明显高于正常组(P<0.05);FZJG中剂量组NAFLD活动度积分与模组比较则明显降低(P<0.05)。PPAR-α蛋白和mRNA表达均明显低于正常组(P<0.05);FZJG高、中、低剂量组PPAR-α蛋白和mRNA表达均高于模型组,但只有FZJG中剂量组PPAR-α蛋白和mRNA表达相对模型组有统计学意义(P<0.05)。结论:复方楂金颗粒剂能明显增强NASH大鼠肝组织PPAR-α的基因表达,抑制脂质在大鼠肝脏内沉积,防治NASH形成及发展。

Objective ： To observe the effect of Fufang Zhajin Granule （FZJG） on peroxisome proliferator - activated receptor- ct（ PPAR - ct）expression of rats with non - alcoholic fatty liver disease （NAFLD） for ap- proaching the possible mechanism . Methods： SD rats were randomly divided into six groups： normal control group（ normal diet）, rats in the model group , positive and FZJG low, middle, high dose groups were given high fat diet to induce steatohepatitis for 10 weeks respectively, then each group was given saline, polyene phosphati- dylcholine, high , medium , low dose FZJG for 10 weeks respectively. Then rats were sacrificed, the following tests were performed： Liver index, histopathological changes in the liver and hepatic PPAR - ctmRNA and protein ex- pression leves（ PT- PCR and immunohistochemical methods）. Rusults： The liver index of the model group was significantly higher than that of the normal group（P 〈 0. 01 ） ;the liver index of FZJG middle dose group and low dose group was lower than that of model group, which was statistically significant（ P 〈 0. 05, P 〈 0.01 ）. The NAFLD activity score in the model group was significantly higher than that in the normal group （ P 〈 0. 05 ）, NAS in FZJG middle dose group was significantly lower than that in model group （ P 〈 0. 05 ）. The PPARa protein and mRNA expressions in model group were significantly lower that those in normal （ P 〈 0, 05 ） ; which in FZJG high, middle and low dose groups were increased obviously, the difference between middle dose group and model was significant（ P 〈 0. 05）. Conclusion： FZJG can significantly enhance PPAR - a expression in the liver tissue of rats with NASH , repress fat deposition in rats＇ livers , prevent and cure NASH.