Study of genetic evolution of oil inclusion and density of surface oil by measurement of fluorescence lifetime of crude oil and oil inclusion

By using fluorescence lifetime image microscope （FLIM） and time-correlated single photon counting （TCSPC） technique, we measured fluorescence lifetime of crude oils with density of 0.9521-0.7606 g/cm3 and multiple petroleum inclusions from Tazhong uplift of Tarim Basin. As indicated by the test results, crude oil density is closely correlated with average fluorescence lifetime following the regression equation Y=-0.0319X＋0.9411, which can thus be used to calculate density of oil inclusions in relation to fluorescence lifetime and density of corresponding surface crude. For type A oil inclusions showing brown-yellow fluorescence from Tazhong 1 well in Tarim Basin, their average fluorescence lifetime was found to be 2.144-2.765 ns, so the density of surface crude corresponding to crude trapping these oil inclusions is 0.852-0.873 g/cm3, indicating that they are matured oil inclusions trapped at earlier stage ofoil formation. For type B oil inclusions with light yellow-white fluorescence, their average fluorescence lifetime was found to be 4.0294.919 ns, so the density of surface crude corresponding to crude trapping these oil inclusions is 0.784-0.812 g/cm3, indicating that they are higher matured oil inclusions trapped at the second stage of oil formation. For type C oil inclusions showing light blue-green fluorescence, their average fluorescence lifetime was found to be 5.0634.168 ns, so the density of surface crude corresponding to crude trapping these oil inclusions is 0.743-0.779 g/cm3, indicating that they are highly-matured light oil inclusions trapped at the third stage of oil formation.

By using fluorescence lifetime image microscope(FLIM)and time-correlated single photon counting(TCSPC)technique,we measured fluorescence lifetime of crude oils with density of 0.9521-0.7606 g/cm^3 and multiple petroleum inclusions from Tazhong uplift of Tarim Basin.As indicated by the test results,crude oil density is closely correlated with average fluorescence lifetime following the regression equation Y=-0.0319X+0.9411,which can thus be used to calculate density of oil inclusions in relation to fluorescence lifetime and density of corresponding surface crude.For type A oil inclusions showing brown-yellow fluorescence from Tazhong 1 well in Tarim Basin,their average fluorescence lifetime was found to be 2.144-2.765ns,so the density of surface crude corresponding to crude trapping these oil inclusions is 0.852-0.873 g/cm^3,indicating that they are matured oil inclusions trapped at earlier stage of oil formation.For type B oil inclusions with light yellow-white fluorescence,their average fluorescence lifetime was found to be 4.029-4.919 ns,so the density of surface crude corresponding to crude trapping these oil inclusions is 0.784-0.812 g/cm^3,indicating that they are higher matured oil inclusions trapped at the second stage of oil formation.For type C oil inclusions showing light blue-green fluorescence,their average fluorescence lifetime was found to be 5.063-6.168 ns,so the density of surface crude corresponding to crude trapping these oil inclusions is 0.743-0.779g/cm^3,indicating that they are highly-matured light oil inclusions trapped at the third stage of oil formation.