一种复合生物消毒剂的研制

Development of compound bio-disinfector

目的 :研制一种安全性高的复合型生物消毒剂。方法 :筛选安全性高的生物消毒剂,进行最小杀菌浓度(minimum bactericidal concentration,MBC)测试,选择消毒性高的消毒剂,并辅以合适的表面活性剂和助剂,通过正交设计,确定高效的生物消毒剂配方。参考《消毒技术规范》的规定对不同配方进行消毒效果评价;将消毒剂配方于54℃保存3个月,测试其长期保存后的消毒效果。结果:获得了组分为5 mg/mlε-聚赖氨酸、1%甜菜碱表面活性剂(BS-12)、5 mg/ml甘氨酸和0.2%甘油的复合型生物消毒剂配方,其在3 min内对大肠杆菌、金黄色葡萄球菌、绿脓杆菌的消毒对数值均大于4,消毒效果理想。加速试验结果表明,该消毒剂放置2 a消毒效果保持不变。结论:该生物消毒剂具有优良的消毒效果,可以用于物体表面等的消毒。

Objective To prepare a compound bio-disinfector with high safety. Methods Minimum bactericidal concentration（MBC） test was used to select a high-efficiency disinfector from several high-safety ones, and then proper surfactant and promoter as well as orthogonal design were involved in to determine the bio-disinfector remedies. The efficacies of bio-disinfectors with different remedies were evaluated according to Disinfection Technical Specifications. The prepared disinfector underwent 3-month storage at 54 ℃, and then was tested on disinfection efficacy after long-term storage. Results The developed disinfector involved in 5 mg/ml ε-polylysine, 1% BS-12, 5 mg/ml Gly and 0.2% glycerol, which had the logarithm value higher than 4 when used to disinfect escherichia coli, staphylococcus aureus pseudomonas aeruginosa.Accelerated test proved the prepared disinfector was still efficient after 2-year storage. Conclusion The disinfector behaves well in the disinfection of surface and etc.