菟丝子水提液对环磷酰胺致小鼠肝脏损伤的保护作用

Protective effect of water extract of Semen cuscutae on cyclophosphamide-induced liver injury in mice

为了探讨菟丝子水提液对环磷酰胺(CP)所致小鼠肝脏损伤的保护作用,试验对SPF级KM雄性小鼠腹腔注射环磷酰胺制作肝脏损伤模型,再将小鼠分为CP对照组、阴性对照组及高、中、低菟丝子水提液药物组,给高、中、低菟丝子水提液药物组小鼠灌胃菟丝子水提液,观察菟丝子水提液对环磷酰胺所致肝脏损伤的肝脏系数、肝脏形态的影响。结果表明:与CP对照组比较,高、中、低菟丝子水提液药物组均可显著提高小鼠肝脏系数(P<0.05);同时高、中、低菟丝子水提液药物组均有改善肝脏组织结构,减少肝细胞坏死数量,促进肝细胞再生等作用,其中高菟丝子水提液药物组改善效果较明显。说明菟丝子水提液对环磷酰胺所致肝脏损伤有保护作用。

To investigate the protective effect of water extract of Semen cuscutae on cyclophosphamide （CP） - induced liver injury in mice. The SPF KM male mice were administered with cyclophosphamide by intraperitoneal injection to make a liver injury model. The mice were randomly divided into the CP control group, negative control group, and the high -, middle -, and low - dose groups of water extract of Semen cuscutae. The mice in the high - , middle - , and low - dose groups of water extract of Semen cuscutae were intragastrically administrated to observe the effect of the water extract of Semen cuscutae on liver coefficients and liver morphology of cyclophosphamide - induced liver injury in the mice. The results showed that the liver coefficients of mice in the high -, middle -, and low - dose groups of water extract of Semen cuscutae were all significantly improved （P 〈0.05） compared with those in the CP control group. Meanwhile, the hepatic tissue structure was improved some- what, and the number of necrosis of liver ceils was reduced, and liver cell regeneration was observed in the high -, middle -, and low - dose groups of water extract of Semen cuscutae. Thereinto, the improved effect of the high dose group of water extract of Semen cuscutae was more obvi- ous. The results indicate that the water extract of Semen cuscutae has the protective effect on cyclophosphamide - induced liver injury in mice.