核转录因子-κB在膀胱癌组织中的表达及其临床意义

Clinical significance of nuclear transcription factors kappa B expression in bladder cancer

目的探讨核转录因子-κB(NF-κB)在膀胱癌组织中的表达及其与患者临床病理特征和预后的相关性。方法选取98例膀胱癌患者为研究对象,用免疫组化法检测膀胱癌组织、癌旁组织及远癌组织中NF-κB的表达水平,并分析其与患者临床病理特征(包括年龄、性别、病理分级、临床分期及淋巴结转移等)的关系。比较NF-κB阳性组和NF-κB阴性组的术后肿瘤复发风险有无差异。结果与NF-κB在癌组织的阳性表达率61.22%(60/98例)相比,NF-κB在癌旁组织、远癌正常组织的阳性表达率分别为18.37%(18/98例)和5.10%(5/98例),差异均有统计学意义(P<0.05)。NF-κB阳性组肿瘤分化程度G1,G2和G3级分别为9例(15.00%),27(45.00%)例和24例(40.00%);NF-κB阴性组肿瘤分化程度G1,G2和G3级分别为18例(47.37%),15例(39.47%)和5例(13.16%)。NF-κB阳性组和NF-κB阴性组的中位肿瘤复发时间分别为10.10,23.50个月,3年复发风险分别为89.62%和64.19%,差异均有统计学意义(P<0.05)。结论 NF-κB与膀胱癌的恶性程度有关,可作为判断复发的分子标志物。

Objective To evaluate the association between clinical characteristics and nuclear transcription factors kappa B（ NF- κB） expression in bladder cancer. Methods Ninety- eight bladder cancer patients were included in study. The NF- κB expression in cancer tissue,paraneoplastic tissue and normal bladder tissue was examined by immunohistochemical method. The association between clinical characteristics such as age,gender,grade clinical stage,lymph node metastasis and nuclear transcription factors NF- κB expression in bladder cancer was evaluate.The tumor recurrence was assessed between the NF- κB positive and negative group. Results NF- κB positive rate were 61. 22%（ 60 /98cases）,18. 37%（ 18 /98 cases） and 5. 10%（ 5 /98 cases） in cancer tissue,paraneoplastic tissue and normal bladder tissue respectively with statistical difference（ P〈0. 05）; G1,G2 and G3 differentiation distribution were 9 cases（ 15. 00%）, 27 cases（ 45. 00%）, 24 cases（ 40. 00%） in NF- κB positive expression group and 8 cases（ 47. 37%）,15 cases（ 39. 47%）,5 cases（ 13. 16%） in NF- κB negative expression group respectively which indicated significant differencebetween tumor differentiation in NF- κB positive and negative group（ P〈0. 05）. The median recurrence time were10. 10 and 23. 50 months for the NF- κB positive and negative group with the 3- year recurrence risk of 89. 62% and64. 19% respectively which indicated NF- κB positive group had more risk for recurrence hazard ratio（ P〈0. 05）.Conclusion NF- κB is associated with pathology grade of bladder cancer which could be used as a biomarker for judging recurrence of bladder cancer.