硫化氢在模拟正畸力作用下对牙周膜细胞Runx2表达的影响

Effect of hydrogen sulfide on osteogenic Runx2 expression of human periodontal ligament cells under orthodontic force

目的 模拟正畸力作用下,观察不同浓度硫化氢（Hydrogen Sulfide,H2S）对人牙周膜细胞成骨相关因子Runx2表达的影响.方法 对人牙周膜细胞分别施加5％循环张力0、6、12、24、48h,通过Real-time PCR及Western blot检测成骨因子Runx2的基因及蛋白表达;观察加力与不加力时不同浓度H2S对Runx2基因表达的影响.并通过Western blot检测p38-MAPK信号蛋白的变化.结果 加力后Runx2基因表达升高,在加力24 h时最高;不论加力与否,H2S浓度为50 μM时促进成骨因子Runx2升高作用最明显;加力24 h,H2S浓度为50 μM时,检测信号蛋白p38-MAPK磷酸化水平明显升高.结论 在模拟正畸力作用下,H2S可引起人牙周膜细胞成骨因子Runx2表达升高,并且p38-MAPK信号通路参与H2S引起人牙周膜细胞成骨因子Runx2表达升高这一过程.

Objective To explore the effects of Hydrogen Sulfide （H2S） with different concentrations on the expression of osteogenic Runx2 in the periodontal tissue under orthodontic force.Methods Human periodontal ligamentcells （hPDLCs） were applied with cycle tension force for 6 h,12 h,24 h,48 h.The expression of Runx2 was investigated though real-time PCR and western blot.Then,signal-regulated kinases p38-MAPK activities under H2S treatment were measured though western blot.Results Tension stimulation promoted the expression of mRNA and proteinof Runx2 in hPDLCs,which were highest after 24h force application.50μm H2S can promote the expression most,with or without force application.Importantly,p38-MAPK wasobviously activated upon induction by 50μm H2 S and 24 h force application.Conclusions H2 S could promote the expression of Runx2 of hPDLCs by activating p38-MAPK signaling pathwaysunder proper tension stimulation.