磷酸烯醇式丙酮酸羧激酶基因启动子-232多态性与动脉硬化性脑梗死关系的研究

Study on the relationship between the polymorphism of phosphoenolpyruvate carboxykinase gene promoter-232 and atherosclerotic cerebral infarction

目的 探讨磷酸烯醇式丙酮酸羧激酶基因（PCK1）启动子-232位点基因多态性与动脉硬化性脑梗死的关系。方法 选择2012年5月-2013年2月在广州医科大学附属第一医院（以下简称“我院”）神经内科住院的动脉硬化性脑梗死患者128例为脑梗死组,同时选择在我院体检中心体检的70名健康成人为对照组。采用聚合酶链反应-限制性片段长度多态性方法,进行PCK1启动子-232基因多态性分析;测定血糖、血脂;颈动脉超声多普勒检查颈总动脉内膜中层厚度（IMT）及颈动脉斑块。结果 脑梗死组GC、GG基因型餐后2 h血糖及低密度脂蛋白胆固醇水平均高于CC基因型,差异有统计学意义（P〈0.05）。脑梗死组GC、GG基因型及G等位基因频率明显高于对照组（P〈0.05）。脑梗死组GG＋GC基因型及G等位基因在易损斑块和非易损斑块中的分布情况比较,差异有统计学意义（P〈0.05）。两组不同基因型之间左、右侧颈总动脉IMT比较,差异无统计学意义（P〉0.05）。结论 PCK1启动子-232基因多态性与广东地区汉族人群动脉硬化性脑梗死的关系密切,G等位基因可能是脑梗死的易感因素。

Objective To study the relationship between the polymorphism of phosphoenolpyruvate carboxykinase （PCK1） gene promoter-232 and atherosclerotie cerebral infarction. Methods 128 patients with atherosclerotic cerebral infarction admitted in Department of Neurology, the First Affiliated Hospital of Guangzhou Medical University （＂our hospital＂ for short） from May 2012 to February 2013 were selected as cerebral infarction group, and 70 healthy a.dults were selected as control group in the physical examination center of our hospital. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to analyze the PCK1 promoter-232 gene polymorphism. The blood glucose and plasma lipids were measured. The carotid intima-media thickness （IMT） and CAP were detected with carotid uhrasonagraphic Doppler. Results Postprandial 2 h blood glucose and low density lipoprotein cholesterol levels of GC, GG genotype in cerebral infarction group were significantly higher than those of CC genotype, the differences were statistically significant （P 〈 0.05）. The ratio of GC, GG genotype and G allele in cerebral infarction group were significandy higher than those in control group （P 〈 0.05）. The distribution of GG＋GC genotype and G allele in vulnerable plaque and vulnerable plaque of cerebral infarction group were compared, the differences were statistically significant （P 〈 0.05）. IMT of left and right carotid artery different genotypes in two groups were compared, with no statistical difference （P 〉 0.05）. Conclusion PCK1 promoter-232 gene polymorphism is closely correlated with atherosclerotic cerebral infaetion, G allele may be a predisposing factor for cerebral infarction.