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Conventional and Molecular Detection of Plasmodium in Domestic Poultry Birds

Conventional and Molecular Detection of Plasmodium in Domestic Poultry Birds
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摘要 The present study was conducted to detect the Plasmodium, the causative agent of malaria in domestic poultry birds. Blood smear method was used as the conventional method for the detection, whereas the polymerase chain reaction (PCR) was performed for further confirmation. A total of 50 blood samples were collected from poultry birds showing the malarial symptoms. The results of blood smear methods showed two samples (4%) were infected with genus Plasmodium, whereas the PCR analysis showed four (8%) positive samples. These results confirm that the PCR is more sensitive method for detecting the Plasmodium when compared with conventional methods, and the microscopy diagnosed 50% false negative results that were confirmed by PCR.
出处 《Journal of Agricultural Science and Technology(A)》 2016年第4期283-289,共7页 农业科学与技术(A)
关键词 POULTRY PLASMODIUM polymerase chain reaction microscopy. 疟原虫属 分子检测 家禽 PCR分析 聚合酶链反应 血液样本 检查结果 禽流感
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