辛伐他汀对2型糖尿病动脉粥样硬化大鼠血管内皮的影响研究

Effect of Simvastatin on Vessel Endothelium in Rats with Type 2 Diabetic and Atherosclerosis

目的探讨辛伐他汀对2型糖尿病动脉粥样硬化大鼠血浆血管内皮生长因子(VEGF)、脂肪因子C1q/TNF相关蛋白:3(CTRP3)水平的影响。方法 2014年1月—2016年7月,选取SPF级SD大鼠40只,采用随机数字表法,将大鼠随机分为对照组(n=20)和辛伐他汀组(n=20)。采用单次腹腔注射链脲佐菌素溶液混合维生素D3及高脂饮食饲养建立2型糖尿病动脉粥样硬化大鼠模型。对照组大鼠以20 ml·kg^(-1)·d^(-1)蒸馏水灌胃,辛伐他汀组采用辛伐他汀溶液20 mg·kg^(-1)·d^(-1)灌胃,1次/d,连续灌胃7 d。治疗后测定血脂、VEGF、CTRP3水平。结果辛伐他汀组治疗后TC、LDL水平低于对照组,TG、HDL水平高于对照组(P<0.05)。辛伐他汀组治疗后血浆VEGF、CTRP3水平低于对照组(P<0.05)。HE染色显示,辛伐他汀组大鼠动脉内膜光滑、连续,各层分界清楚,结构完整,未见脂质沉积;对照组大鼠可见少量泡沬细胞和粥样斑块。结论 2型糖尿病动脉粥样硬化大鼠给予辛伐他汀治疗效果理想,能降低血浆VEGF、CTRP3水平。

Objective To investigate the effect of simvastatin on the level of VEGF and CTRP3 in the rats with type 2 diabetic atherosclerosis. Methods From January 2014 to July 2016 , 40 SD rats were selected and randomly divided into control group （n = 20） and simvastatin group （n =20） by using the method of random number table. Type 2 diabetic atherosclerosis model in rats was established by intraperitoneal injection of urea solution with a mixture of vitamin D3 and high fat diet. Rats in control group were fed with 20 ml · kg^-1 · d^-1 distilled water, simvastatin group was treated with simvastatin solution 20 mg ·kg^-1 · d^-1 , 1 time/d, continuous irrigation of 7 days. Blood lipid, VEGF and CTRP3 levels were determined after treatment. Results After treatment, TC, LDL levels in simvastatin group were lower than those in the control group, TG, HDL levels were higher than those in the control group, and VEGF, CTRP3 levels were lower than those in the control group （P 〈0. 05）. HE staining showed that the intima of the simvastatin group was smooth and continuous, the boundaries were clear, the structure was complete, and there was no lipid deposition; a small amount of foam cells and atherosclerotic plaque were found in the control group. Conclusion Simvastatin used for type 2 diabetic atherosclerosis rats is ideal , and can reduce the plasma VEGF, CTRP3 levels.