大鼠Walker-256移植性睾丸恶性肿瘤模型的建立及动态观察

Established and Dynamically observed The Rat Transplanted Testicular Malignant Tumor Models With Walker-256 cells

目的研究大鼠Walker-256细胞移植性睾丸恶性肿瘤的成模性及稳定性。方法取健康SD大鼠25只,采用睾丸注射方法,直视下将Walker-256肿瘤细胞悬液注射在大鼠睾丸内,分别在接种肿瘤后7,14,21,28,35d行彩超检查,检查后麻醉处死,每次5只,行大体解剖以及病理学检查,以此观察移植性睾丸恶性肿瘤模型的成模率、肿块大小、其他脏器转移情况及声像图特点。结果大鼠睾丸恶性肿瘤均种植成功,于接种肿瘤组织21-28d后,大鼠开始出现轻度体重减轻、纳差以及腹水等体征,35d后大鼠开始逐渐出现死亡;超声显示移植14d睾丸见低回声包块,21d睾丸内见肿瘤边缘血供丰富。病理组织学可见肿瘤细胞呈巢状或条索状弥漫分布,形态不规则,细胞异型性大,曲精小管等正常组织结构破坏、坏死。结论采用Walker-256能成功建立移植性睾丸恶性肿瘤模型,成瘤率高,该肿瘤模型的生物学行为与临床病程相似,可用于抗肿瘤疗效评价。

Objective To establish the transplanted testicular malignant tumor models with Walker-256 cells and observe its feasibility and stability. Methods Twenty-four SD rats were injected Walker-256 cells in testis respectively and then at the 7,14,21,28,35 days after inoculated tumor,using colour Doppler ultrasound to check. Then anesthesia executed to anatomy and pathological exam,observing the portability of testicular malignant tumor model,tumor size,and tumor metastasia. Results 21-28 days after inoculation,the rats body weight were slightly decreased,poor appetite,and ascites were observed. Then 35 days after inoculation,The rats began to die. Ultrasound showed the low echo mass after 14 d testicular transplanted and then at the 21 th day,the blood flow was abundant around the tumor. Pathological exam showed the tumor cells diffused distribution in nests or irregular shape,and the cells were atypia. The structures of seminiferous tubule were damaged and necrosis. Conclusion Walker-256 can successfully establish testicular malignant tumors model,which the biological behavior was similar to clinical course. This model can be used in the evaluating the anti-tumor efficacy.