摘要
针对免疫磁珠技术中磁珠大小对免疫检测结果的影响问题,以三种免疫磁珠为研究对象,分析了单个细胞结合不同大小磁珠的数量情况,利用流式细胞术分析了γ-H2AX相对荧光强度变化与辐射剂量的变化关系,探讨了免疫磁珠与细胞结合对紫外线UVC诱发CD4细胞γ-H2AX相对荧光强度变化的影响。发现细胞与磁珠比为1∶10时单个细胞至少可以结合一个磁珠,结合数量与磁珠大小有关;UVC辐照引起CD4细胞γ-H2AX相对荧光强度变化与辐射剂量呈线性正相关,小磁珠(50 nm)和中磁珠(2.8μm)与细胞结合γ-H2AX荧光强度与辐射剂量之间仍具有一定的剂量效应关系,但大磁珠(4.5μm)与细胞结合会导致γ-H2AX相对荧光强度与辐射剂量的线性关系不明显。结果可为今后将免疫磁珠用于辐射诱导的DNA损伤评估奠定基础。
The size of the beads has an impact on the results of the immunoassay in immunomagnetic bead technique. In this study, three kinds of immunomagnetic beads were selected as the research objects. The number of individual cells combined with the beads was analyzed, the relationship b e-tween the relative fluorescence intensity changes of Y - H 2AX and the radiation dose w a s studied by flow cytometry, and the effects of immunomagnetic beads on UVC - induced relative fluorescence in-tensity changes of Y - H 2AX in CD 4 cells were discussed. It w a s found that the single cell could c o m-bine at least one bead when the ratio of cells and magnetic beads was 1 ： 10. T h e n u m b e r of beads on the cells was dependent on the size of the beads. U V C - i n d u c e d changes in the relative fluorescence intensity of Y - H 2AX were linearly correlated with the radiation dose. In the cases of small beads （50 nm ） and medium beads （2.8 pm ） , there w a s dose-effect relationship between the Y- H 2A X fluores-cence intensity and the radiation dose. While for the magnetic beads （4.5 pm ） combined with the cells, the linear correlation between the relative fluorescence intensity of Y- H 2 A X and the radiation dose was not obvious. These results m a y be helpful for the application of immunomagnetic beads in DNA damage assessment induced by radiation.
出处
《载人航天》
CSCD
2017年第1期114-117,142,共5页
Manned Spaceflight
基金
中央高校基本科研业务费专项资金(3132014306)
中国科学院空间科学战略性先导科技专项(XDA04020202-12)
