MALDI-TOF MS在细菌耐药性分析中的临床应用

MALDI-TOF MS in the determination of drug resistance

基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)具有快速、高通量、高准确性和低成本等特点,为临床微生物的鉴定带来了革命性的变化,也给细菌耐药性分析提供了一条新思路。常规细菌耐药性检测方法有纸片法、稀释法、E-test等,这些方法均需要长时间的培养,不能满足临床对快速药物敏感性指导的需求。MALDI-TOF MS用于细菌耐药性的检测具有巨大的应用价值与前景。已有一些关于MALDI-TOF MS在细菌耐药性检测的实验和临床应用的报道。文章就近年来MALDI-TOF MS在细菌耐药性分析相关的病原体种类、检测方法和检测效果等方面作一综述。

The application of matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF MS ） has brought a revolutionary change for microbial identification program in clinical microbiological laboratories, for its fast, high- throughput, accuracy and cost- effectiveness. Furthermore, MALDI- TOF MS provides a new way for drug resistance determination. It is time-consuming for commonly-used methods of routine drug resistance determination, such as Kirby- Bauer, dilution and E- test. These methods can not meet the clinical demand for quick selection of sensitive drugs. So, the application of MALDI-TOF MS for the determination of drug resistance has research value and prospect. Some experiments and clinical application of MALDI-TOF MS for determining drug resistance have been reported. This review introduces the types of pathogens, determination methods and the efficiency of MALDI-TOF MS for determining drug resistance in recent years.