摘要
研究金娃娃萱草的组织培养及植株再生.以MS为基本培养基,离体培养金娃娃萱草新生侧苗的根状茎.研究结果表明:芽诱导的最佳培养为MS+6-BA 0.5mg·L^(-1)+NAA 0.2mg·L^(-1),芽诱导效率可达100%;最佳生根培养基为加入0.2 mg·L^(-1)NAA的1/2MS培养基,生根率达100%,且根系发达健壮,茎叶生长旺盛.
To study In vitro Culture and Plantlet Regeneration of Hemerocallis fuava. Root-like stem explant was cultured in vitro on MS medium. The results showed that the optimum medium of inducing cluster buds was MS + 6-BA 0. 5mg . L-1+NAA 0. 2 mg . L-1 ,the efficiency of in-ducing buds was 100 %. The 1/2 MS medium with addition of 0. 2 mg . L-1 NAA was the most appropriate for inducing test-tube plantlet to produce roots and the rate of rooting might reach 100 % ,and the root system developed robust, stem and leaf grew well.
出处
《牡丹江师范学院学报(自然科学版)》
2017年第1期49-51,共3页
Journal of Mudanjiang Normal University:Natural Sciences Edition
基金
国家烟草专卖局基因组重大专项[110201501015(JY-02)]
牡丹江师范学院省级重点创新预研项目(SY201229)
牡丹江市科学技术计划项目(Z2013n020)
关键词
金娃娃萱草
组织离体培养
植株再生
Hemerocallis fuava
in vitro tissue culture
plantlet regeneration