金娃娃萱草的离体培养及植株再生

In vitro Culture and Plantlet Regeneration of Hemerocallis fuava

研究金娃娃萱草的组织培养及植株再生.以MS为基本培养基,离体培养金娃娃萱草新生侧苗的根状茎.研究结果表明:芽诱导的最佳培养为MS+6-BA 0.5mg·L^(-1)+NAA 0.2mg·L^(-1),芽诱导效率可达100%;最佳生根培养基为加入0.2 mg·L^(-1)NAA的1/2MS培养基,生根率达100%,且根系发达健壮,茎叶生长旺盛.

To study In vitro Culture and Plantlet Regeneration of Hemerocallis fuava. Root-like stem explant was cultured in vitro on MS medium. The results showed that the optimum medium of inducing cluster buds was MS ＋ 6-BA 0. 5mg . L-1＋NAA 0. 2 mg . L-1 ,the efficiency of in-ducing buds was 100 %. The 1/2 MS medium with addition of 0. 2 mg . L-1 NAA was the most appropriate for inducing test-tube plantlet to produce roots and the rate of rooting might reach 100 % ,and the root system developed robust, stem and leaf grew well.