IL-22、IL-27、VEGF、TF及TFPI对良恶性浆膜腔积液的鉴别价值

The Diagnostic Value of IL-22,IL-27,VEGF,TF and TFPI in Benign and Malignant Pleural Effusion

目的评估浆膜腔积液中白介素22(interleukin-22,IL-22)、IL-2 7、血管内皮生长因子(vascular endothelial growth factor,VEGF)、组织因子(tissue factor,TF)和组织因子途径抑制物(tis sue factor pathway inhibitor,TFPI)水平在鉴别良恶性浆膜腔积液中的价值。方法应用酶联免疫吸附测定(E LISA)法检测41例病理学和影像学检测确诊为恶性浆膜腔积液患者、41例良性浆膜腔积液患者的浆膜腔积液中IL-22、IL-27、VEGF、TF和TFPI水平,并加以分析。结果恶性浆膜腔积液患者的浆膜腔积液中IL-22、IL-27、VEGF、TF和TFPI水平明显高于良性浆膜腔积液患者,差异均具有统计学意义(P<0.05)。ROC分析表明,恶性浆膜腔积液中IL-22、IL-27、VEGF、TF和TFPI的ROC曲线下面积分别为0.638、0.750、0.687、0.568和0.657。而联合这5个指标建立的预测诊断模型,ROC曲线下面积为0.793。结论浆膜腔积液中IL-22、IL-27、VEGF、TF和TFPI水平对鉴别良恶性浆膜腔积液有重要参考价值。

Objective To investigate the diagnostic value of IL-22,IL-27,VEGF,TF and TFPI inhibitor value in the benign and malignant pleural effusions. Method Selected ninety pleural patients were subdivided into two groups according to the Results of pathology and iconography diagnosis,ie the benign pleural effusions （benign） group with 43 cases and malignant pleural effusions （malignant） group with 47 cases. To test the diagnostic ability of several indicators used alone and in combination,the concentrations of IL-22,IL-27,VEFG,TF and TFPI were determined by ELISA Method,and the concentrations of these five biomarkers in pleural effusion were compared,then receiver operating curve （ROC） analysis was performed. Results A significant difference of IL-22,IL-27,VEGF,TF and TFPI levels were observed in patients with malignant and benign pleural effusion （P〈0.05）. Receiver operating curve analysis indicated that several indicators used alone showed a less potent diagnostic ability of benign and malignant pleural effusion,the area under the ROC curve of less than 0.8; while these biomarkers used in combination showed an obvious improvement in sensitivity,specificity and accuracy. Conclusion The combination of several biomarkers can provide a reference for the differential diagnosis of benign and malignant pleural effusion. Because of the high accuracy,efficiency and simplicity,this Method is worthy promotion.