低频脉冲电磁场对C2C12细胞增殖过程中Pax7表达的影响

Effects of low - frequency pulsed electromagnetic fieldson Pax7 expression in proliferation of C2C12 muscle satellite cells

目的：探讨低频脉冲电磁场（PEMF）在肌卫星细胞C2C12增殖过程中对Pax7表达与合成的影响作用。方法培养C2C12细胞，将传代培养好的细胞分为对照组，0.5 mT磁场组，1.0 mT磁场组，1.5 mT磁场组4组，进行连续5 d内培养，同时分别于第1、2、3、4、5 d内细胞送检，用免疫化学染色法检测细胞表型的变化、用PCR、Western印迹检测细胞中Pax7的表达和合成水平。结果与对照组（0.45±0.07）相比较，不同强度低频脉冲电磁场能够诱导肌卫星细胞C2C12系体外细胞培养增殖过程中Pax7的表达，其中第4 d 0.5 mT组表达Pax7 mRNA（8.51±0.16）最强，P〈0.05，差异有统计学意义。结论在一定的条件下，不同强度低频脉冲电磁场在肌卫星细胞C2C12系体外细胞培养增殖过程中对Pax7 mRNA的表达有诱导促进作用，进而参与肌卫星细胞增殖分化过程。

Objective To investigate the effects of low-frequency pulsed electromagnetic fields （PEMFs）onthe expressionand synthesis of paired-box transcription factor7（Pax7）in the proliferation of C2C12 muscle satellite cells. Methods C2C12 cells were cultured by cell culture. The subcultured cells were divided into the control group,0.5 mT magnetic field group,1.0 mT magnetic field group and 1.5 mT magnetic field,and cultured for continuous 5 d. All groups were examined at 1,2,3,4,5 d,respectively. Immunohistochemical staining was used to determine the changes of cell phenotype. The synthesis and expression levels of Pax7 were determined by PCR,Western-blot. Results Compared with the control group （0.45 ± 0.07）,different intensities of low-frequency PEMFs could induce the Pax7 expression in the proliferation of the in vitro cultured C2C12 muscle satellite cells. Among the four groups,the Pax7 mRNA expression at 4 d in the 0.5 mT group（8.51 ± 0.16） was the highest,and the difference was statistically significant（P〈0.05）. Conclusion Under certain conditions,different intensitiesof low-frequency PEMFs can induce the Pax7 mRNA expression in the proliferation of the in vitro cultured C2C12 muscle satellite cells,and further involves in the proliferation and differentiation of muscle satellite cells.