平阳霉素诱导花生胚小叶再生体系的抑制探析

Discussion on Inhibition of Pingyangmycin(PYM) on Induction Regeneration System of Peanut Embryo Leaflet

[目的]运用组织培养的方法对花生胚小叶（种胚）进行再生体系繁殖。[方法]以平阳霉素（PYM）作为化学诱变剂,进行花生不定芽诱变。[结果]结果表明,PYM对诱导体胚发生有显著的抑制作用。PYM浓度过高,体胚（新个体）诱导率显著下降,外植体褐化率急剧上升,过低愈伤组织诱导率明显升高。[结论]确定阜花16、阜花18适宜的PYM离体诱变浓度为0.75~1.0 mg/L,诱变培养时间为28 d。

[Objective] The aim was to study regeneration system reproduction of peanut embryo leaflets（embryos）, by using tissue culture method. [Method] The adventitious bud mutation of peanut was conducted, by taking Pingyangmycin（PYM） as a chemical mutagen. [Result] The results showed that PYM significantly inhibited the induction of somatic embryogenesis. The induction rate of somatic embryos（new individuals） was significantly decreased with high PYM concentration, and the browning rate of explants increased sharply. But callus induction rate was significantly higher when Pingyangmycin concentration was too low. [Conclusion] The suitable mutagenic concentration of PYM was 0.75-1.0 mg/L for Fuhua 16, Fuhua 18, and the mutagenic culture time was 28 days.