敲低趋化因子受体7(CCR7)抑制MG63骨肉瘤细胞的增殖和侵袭并诱导其凋亡

CCR7 silence by siRNA inhibits proliferation,invasion and promotes apoptosis of human MG63 osteosarcoma cells

目的研究小干扰RNA(siRNA)靶向抑制趋化因子受体7(CCR7)表达对MG63人骨肉瘤细胞增殖、侵袭及凋亡的影响。方法设计并合成靶向抑制CCR7表达的siRNA,转入MG63细胞后,采用Western blot法检测CCR7蛋白水平,异硫氰酸荧光素标记的膜联素Ⅴ/碘化丙啶(annexinⅤ-FITC/PI)双标记结合流式细胞术检测细胞凋亡,MTT法检测细胞增殖,TranswellTM侵袭和迁移实验检测细胞侵袭和迁移能力。结果 MG63细胞转染CCR7-siRNA后,细胞CCR7表达明显下调;同时细胞增殖明显减弱,侵袭和迁移能力明显下降,且细胞凋亡率升高。结论下调MG63骨肉瘤细胞的CCR7,可明显抑制细胞增殖、侵袭和迁移,并能诱导细胞凋亡。

Objective To investigate the effect of siRNA-mediated chernokine receptor 7 （CCR7） silence on the proliferation, migration, invasion and apoptosis of human MG-63 osteosarcoma cells. Methods The study designed and synthesized siRNA targeting CCR7 （CCRT-siRNA）. After MG63 cells were transfected with CCRT-siRNA, the expression of CCR7 was identified by Western blotting; cell apoptosis was detected by annexin V-FITC/PI double staining combined with flow cemetery; cell proliferation was tested by MTT assay; and cell migration and invasion abilities were examined by TranswellTM migration/invasion assays. Results CCR7 expression in MG63 cells was significantly inhibited after transfected with CCR7-siRNA. At the same time, cell proliferation, migration and invasion abilities were distinctly suppressed, and cell apoptosis rate increased. Conclusion Down-regulating CCR7 expression in MG63 cells could apparently inhibit cell proliferation, migration and invasion abilities of MG63 cells, and also induce cell apoptosis.